摘要
本研究旨在探讨大蒜素对小鼠脾细胞雌激素和雄激素受体的影响。将健康ICR清洁小鼠,按性别随机分成三组实验组和三组对照组。实验组分别连续灌服大蒜素14、21和28 d,对照组同期分别灌服等量生理盐水。采用RT-PCR法检测雌性各组小鼠脾细胞雌激素受体(ERα和ERβ)的mRNA表达水平和雄性各组小鼠脾细胞雄激素受体(AR)的mRNA水平。实验结果显示,雌性小鼠连续灌服大蒜素第14和21 d时实验组ERαmRNA表达水平明显高于对照组(P<0.05),第14 d时ERβmRNA表达水平明显高于对照组(P<0.05)。雄性小鼠连续灌服大蒜素第14、21和28 d时实验组AR mRNA表达水平均明显高于对照组(P<0.05)。综上提示大蒜素可增强小鼠脾细胞雌激素受体(ERα和ERβ)和雄激素受体(AR)mRNA的表达水平。
The aim of this study was to observe the effects of allicin on estrogen and androgen receptors of mice spleen cells. Healthy clean ICR mice were randomly divided into three experimental groups and three control groups. Experimental groups were consecutively fed with allicin for 14,21 and 28 days, respectively, whereas the control groups were administered with saline over the corresponding period. RT-PCR was used to detect the mRNA expression of the estrogen receptor （ERα,ERβ） in spleen cells of female mice and the androgen receptor （AR） in spleen cells of male mice. The mRNA expression levels of ERa mRNA in 14 and 21 days allicin groups ,the ERβ mRNA expression levels in 14 days allicin group were significantly higher than those of the control group （P 〈 0.05）. The mRNA expression levels of All were raised in experimental male mice as compared with the control group（P 〈 0.05）. Hence, it was concluded that allicin enhanced mRNA expression levels of estrogen receptor （ERa and ERβ） and androgen receptor （AR） in mouse spleen cells.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第6期961-966,共6页
Natural Product Research and Development
基金
安徽省教育厅重点项目(KJ2010A322)
