摘要
应用荧光偏振技术 ,差示扫描量热技术 (DifferentialScanning Calorimetry,DSC) ,傅立叶变换红外光谱技术 (Fourier -transforminfraredspectroscopy,FTIR)等检测手段 ,通过测定磷脂液晶相到六角相(Lα→H Ⅱ)的相变温度来研究不同浓度的海藻糖对水化棕榈油酰磷脂酰乙醇胺(L -α - phosphatidylethanolamine ,β-oleoyl-γ - palmitoyl,POPE)的脂多型性的影响。发现海藻糖存在时 ,在30℃ -70℃温度范围内H Ⅱ 相相变消失 ,表明海藻糖有稳定脂质体于双层相的能力。
In this paper,the effect of trehalose on the polymorphism of hydratedPOPE(L-α-phosphatidylethanolamine,β-oleoyl-γ-palmitoyl) was studied by monitoring the phase transition temperature of hexagonal phase (Tbh). Fluorescence anisotropy, FTIR (Fourier-transform infrared spectroscopy), and DSC(Differential Scanning Calorimetry) were used to measure the Tbh. The experimental results showed that trehalose can inhibit the Lα→HⅡ phase transition of the hydrated POPE,thus stabilized the lipid membrane in a planar form.
出处
《生物物理学报》
CAS
CSCD
北大核心
2001年第1期59-64,共6页
Acta Biophysica Sinica
基金
国家自然科学基金!资助项目 (批准号 :39770181)
