摘要
为构建家蚕蛋白质数据库，达到从蛋白质水平研究基因的表达及表达产物的加工修饰 的目的，采用五龄家蚕体壁、中肠和脂肪为材料获取蛋白质样品，用蛋白质双向电泳技术分 离、纯化蛋白质，对其中的４０个蛋白质斑点进行了氨基酸序列分析及同源性检索，发现其中 ５８．５％的蛋白质与果蝇的有关蛋白质具有较高同源性，３６．５％与其他生物的蛋白质具有较高 同源性，只有５％与家蚕已知蛋白质具有较高同源性。研究发现有２７个蛋白质的Ｎ－末端氨基 酸序列在家蚕中是第一次被检测到，并通过互联网向ＳＷＩＳＳ－ＰＲＯＴ数据库进行了登录。研究 证明利用蛋白质数据库的结果，可以得到基因表达及其产物修饰的信息。
We attempted to construct protein databank of silkworm (Bombyx mori L.) for clarifying the gene expression and post--translational modification. Proteins from silkworm body wall, fat body and middle intestines were separated by two--dimensional electrophoresis. The N-terminal amino acid sequences of 40 proteins and their homology to proteins from other organisms were determined. 58.5% Proteins were high homologous to the already reported proteins in D. melanogaster and 36.5% to those in other organisms. Only 5% of the proteins were homologous to the lnown protein in silkworm. The N--terminal sequences of 27 proteins were first found in silkworm, and all these data were registered in SWISS--PROT through the Internet The results suggested that the information of gene expression and post--translational modification could be obtained by the information of protein databank.
基金
浙江省教委及浙江大学资助项目&&
关键词
家蚕
蛋白质数据库
蛋白质纯化
双向电泳
氨基酸序列
蛋白质同源性检索
基因表达
蛋白质修饰
silkworm Bombyx mori L.
protein databank
Protein Purification
2D-PAGE
amino acid sequence
protein homology search
gene expression
post--translational modification
