摘要
目的:将当归药材用不同溶剂处理,得到当归水提醇沉(SCS)部分、醇提取物(CTW)部分以及挥发油部分,建立当归不同提取部分的HPLC和GC指纹图谱,比较当归不同提取部分中化学成分的差异,为全面评价当归药材的质量提供参考。方法:采用HPLC和GC对10批当归药材的不同提取部分的指纹图谱进行研究。用中药色谱指纹图谱计算机辅助相似性评价系统软件对数据进行分析,并对方法进行评价。结果:建立了10批当归药材不同提取部分的指纹图谱,鉴别了SCS部分的4个成分、CTW部分的7个成分、以及挥发油部分的3个成分。结论:该实验方法准确、可靠、重复性好,建立的当归模式指纹图谱可以全面反映当归药材中化学成分的分布,可以作为当归药材及其制剂质量控制的参考依据。
Objective： Angelica Sinensis Radix was processed with different polarity extraction solvent, and got part SCS, part CTW, and part volatile oil. Then compare the differences in the chemical components in Angelica Sinensis Radix from different part of different origins by establishing HPLC and GC fingerprint, so as to provide the references to holistic evaluate the qualities of Angelica Sinensis Radix. Method： To research the Chromatography Fingerprint of 10 batches of Angelica Sinensis Radix with high performance liquid chromatography （HPLC） and gas chromatography （GC）. The similarity software of Chinese medicine fingerprint of computer aided evaluation system was adopted for the data analysis and the method evaluation. Result ： The Fingerprint of different parts of 10 batches of Angelica Sinensis Radix was established, and firstly identify seven main index components. Conclusion： This method have good accuracy, reliable and repeatability. The mode fingerprint established can fully reflect the distribution of chemical composition in Angelica Sinensis Radix, and can be used as the reference for quality control of Angelica Sinensis Radix and its preparations.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第13期55-60,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十二五"科技支撑计划项目(2011BAI05B02)
甘肃省中药药理与毒理学重点实验室开放课题(ZDSYS-KJ-2013-004)
