摘要
目的探讨轻度认知功能障碍（MCI）患者脑脊液和血清中血红素氧化酶-1（HO-1）的含量变化，及其与简易精神状态量表（MMSE）和长沙版蒙特利尔评估量表（MoCA）的相关性，为早期诊断MCI提供依据。方法选择2011年6月至2013年6月我院住院患者，分为MCI组45例和健康对照组85例，收集两组患者的基本资料并进行MMSE和长沙版MoCA量表评估；采用双抗体夹心酶联免疫吸附法（ELISA）检测并比较两组脑脊液和血清中HO-1的含量。结果MCI组35例平均血清HO1含量（612．52±111．48）ng／L，健康对照组67例（384．16±56．86）ng／L，差异有统计学意义（t=2．03，P=0．045）；MCI组26例平均脑脊液HO-1含量（631．38±32．17）ng／L，健康对照组66例（480．75±17．98）ng／L，差异有统计学意义（t=4．09，P=0．000）。而MCI组、健康对照组血清HO-1含量和脑脊液HO1含量比较差异均无统计学意义（t=0．14、1．61，P=0．887、0．110）。MCI组患者脑脊液和血清中HO-1含量均与MMSE评分呈正相关（r=0．398、0．363 P=0．044、0．032），与MoCA得分相关性无统计学意义（均P〉0．05）；对照组脑脊液和血清中HO—1含量变化均与MMSE评分呈负相关（r=-0．572、-0．285，P=0．000、0．019），脑脊液中HO-1含量与MoCA得分呈负相关（r=-0．440，P=0．000），血清中HO-1含量与MoCA得分相关性无统计学意义（P〉0．05）。两组血清和脑脊液中HO—1含量与年龄的相关性均无统计学意义（均P〉0．05）。结论MCI患者脑脊液和血清HO-1含量均明显高于非MCI者，且HO-1含量变化与MMSE评分成正相关，HO-1升高可提示MCI及认知功能下降，有望成为早期诊断MCI的新的标志物。
Objective To explore the change in heme oxygenase-1 （HO-1） level in cerebrospinal fluid（CSF）and serum in patients with mild cognitive impairment（MCI）, and the correlation between HO-1 and the rating scale, to provide a new marker for the diagnosis of MCI. Methods The HO-1 levels in CSF and serum in 45 MCI patients （MCI group） and 85 normal cases （control group） were analyzed with sensitive enzyme linked immunosorbent assays （ELISA）. MMSE and MoCA scores were evaluated. Results The level of HO-1 was higher in MCI group than in control group both in CSF [（631.38±32.17）vs（480.75±17.98）ng/ L, P〈0.05], and in serum [（612. 52±111. 48） w （384. 16±56.86）ng/ L, P〈0. 053. The MCI and normal people HO-1 level had no significant difference between CSF group and serum group （P〉0.05）. In MCI group, the levels of serum and CSF HO-1 had a positive correlation with MMSE scores （ P〈0.05）, but had no obvious correlation with MoCA scores （P〉0.05）. In the normal group, the level of HOwl was negatively related with MMSE scores in serum and CSF, and with MoCA scores in CSF （P〈0.05）, but no obvious correlation in serum （P 〉0.05）. The levels of serum and CSF HO-1 had no obvious correlation with age in both groups （P〉0.05）. Conclusions HO-1 concentration in both CSF and serum is significantly higher in MCI group than in normal group, and positively related with MMSE score. Thus the increase of HO-1 concentration in both CSF and/or serum might be a new marker for the diagnosis of MCI.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2014年第6期594-597,共4页
Chinese Journal of Geriatrics
关键词
认知障碍
血红素氧化酶
Cognition disorders
Heme oxygenase
