摘要
目的:观察中药"复方鳖甲软肝片方"对TGF-β1诱导的肾间质成纤维细胞(NRK-49F)增殖及分泌细胞外基质的影响。方法:以2 ng/ml hTGF-β1诱导NRK-49F细胞,并以软肝片方大、中、小剂量(分别以7g/kg、3.5 g/kg、1.75 g/kg)药物血清进行干预,分别应用MTT法、ELISA、免疫细胞化学方法,观察肾间质成纤维细胞增殖、细胞上清FN浓度及ColⅠ、ColⅢ的表达。结果:软肝方含药血清对hTGF-β1诱导的细胞增殖无影响;但大、中剂量软肝方能不同程度地降低FN的分泌及ColⅠ、ColⅢ的表达,软肝方大剂量组作用最强(P<0.01)。结论:复方鳖甲软肝片方在一定程度上抑制TGF-β1诱导的肾间质成纤维细胞ColⅠ或ColⅢ的表达,大剂量软肝方作用最强。
Objective：Objective：in vitro,we object the effect of Compound Biejia Ruangan Precription（ CBRP） on renal in-terstitial fibroblast proliferation and excreting extracellular matrix induced by TGF-β1 . Methods：Using method of serological pharma-cology, rat renal interstitial fibroblast （NRK-49F cell） stimulated with TGF-β1, simultaneously 10% different rat serum was add-ed to for 48 h as follows：the normal rat serum（ male SD rats were given physiological saline by gastric gavages） , the low dose CBRP-medicated rat serum （given CBRP 7 g/kg）, the middling dose one（given CBRP 3. 5 g/kg）, the high dose one（given CBRP 1. 75 g/kg）, applying to immunocytochemistry, MTT assay, ELISA, cell proliferation and extracellular matrix such as fibronectin, typeⅠcollagen, typeⅢ collagen were detected. Results：Contrasted with the normal rat serum： rat serum medicated-CBRP had no effect on NRK-49F cell proliferation which induced by TGF-β1 , but in the groups of rat serum medicated- high dose CBRP, the high level of cell secretion fibronectin, collagenⅠor collagenⅢwhich boosted by TGF-β1 had been decreased, especially the group of high dose CBRP had best effect （P〈0. 01）. Conclusion：In some extent CBRP could restrain renal interstitial fibroblast secrete or synthesize extracellular matrix such as FN, Col, ColⅢwhich enhanced by TGF-β1 , especially the high dose CBRP exert preferable effects.
出处
《中国中西医结合肾病杂志》
2014年第5期381-384,I0001,共5页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
国家自然科学基金重点资助项目(No.30130220)及面上项目(No.30873345)
