摘要
目的观察不同浓度黄芩素处理宫颈癌细胞后,细胞外信号调节激酶1/2(ERK1/2)与基质金属蛋白酶2(MMP-2)、MMP-9 mRNA及蛋白水平表达的变化。方法体外培养HeLa细胞株,分为空白对照组、100μmol/L、200μmol/L黄芩素处理组,培养24 h。明胶酶谱法检测上清液中MMP-2、MMP-9的活性变化。反转录PCR法检测ERK1/2及MMP-2、MMP-9的mRNA水平的表达变化。Western blot法检测ERK1/2、p-ERK1/2及MMP-2、MMP-9的蛋白水平的表达变化。结果黄芩素处理24 h后,与空白对照组相比较,MMP-2、MMP-9的活性变化逐渐降低,且呈浓度依赖性(P<0.05)。ERK1/2、p-ERK1/2、MMP-2、MMP-9的mRNA及蛋白表达随着黄芩素浓度的增强逐渐降低(P<0.05)。结论黄芩素通过激活ERK1/2信号通路下调MMP-2、MMP-9的表达。
Objective To observe the changes of mRNA and protein expressions of extracellular signal-regulated kinase 1/2 （ERK1/2） and matrix metalloproteinase 2 （ MMP-2）, MMP-9 in cervical cancer cells after stimulated by baicalein at different concentrations. Methods HeLa cells cultured in vitro were exposed to baicalein （0, 100 and 200 iamol/L） for 2.4 hours. The activity of MMP-2 and MMP-9 was measured by gelatin zymography, mRNA expressions of ERK1/2, MMP-2, MMP-9 were determined by reverse transcription PCR （RT-PCR） and protein expressions of ERK1/2, MMP-2, MMP-9 were detected by Western blotting. Results Compared with the control group, baicalein significantly decreased the activity of MMP-2 and MMP-9 in a concentration-dependent manner （ P 〈 0.05）. The mRNA and protein expressions of ERK1/2, p-ERKI/2, MMP-2, MMP-9 also significantly decreased （ P 〈 0.05）. Conclusion Baicalein can activate ERK1/2 signaling pathway and decreasing the activity of MMP-2 and MMP-9.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2014年第8期798-801,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
四川省科技厅课题基金(09032)
四川省卫生厅课题基金(12193)
关键词
黄芩素
宫颈癌细胞
MMP-2
MMP-9
细胞外信号调节激酶1
2
baicalein
cervical cancer
matrix metalloproteinase2
matrix metalloproteinaseg
phosphorylated extracellular signal-regulated kinase 1/2
