摘要
为筛选p53肿瘤因子表达更强的犬乳腺肿瘤细胞系,从而为乳腺肿瘤相关因子和转移机制的实验研究选择更敏感的细胞系,本实验采用由自然发生乳腺肿瘤病例的原发病灶和转移病灶分离培养的4种细胞(CHMp、CHMm、CIPp和CIPm),选取经传代120代后、生长性状稳定的细胞系作为研究对象,对不同细胞系的性状进行分析,同时利用p53抗体对犬乳腺肿瘤细胞系进行免疫组织化学检测。结果显示:通过消化法培养的细胞,其生长曲线呈"S"型,倍增时间分别为21.3 h、35.3 h、42.3 h和49.1 h;采用MTT检测和软琼脂培养比较分析表明CHMp、CHMm的细胞活力和增殖能力均强于CIPp、CIPm;抗体检测显示,p53抗体在CHMp、CHMm中表达强于CIPp、CIPm,在活性和增殖能力最弱的CIPm细胞中表达强度最弱(p<0.01)。实验结果表明,不同来源的细胞系生物学特性差异较大,在研究乳腺肿瘤相关因子和转移机制的实验中选用CHMp和CHMm细胞系敏感性更高,实验效果会更明显。
To screen the higher level of tumor factors p53 and sensitive cell line for the study of the metastatic mechanism in canine breast tumor, we established 4 cell lines （CHMp, CHMm, CIPp and CIPm） derived from primary lesions as well as derived from the metastatic lesions through primary cultures of tissue from a dog. Then we characterized the cancer cells. The result showed that the growth curve shown ＂S＂ shape accorded with the digestion method in different period. MTT assay and soft agar-agar culture demonstrated that the cell proliferation and vigor of CHMp and CHMm were high compared with the other two groups; Immunohistochemical technique showed the expression of p53 in CHMp and CHMm were higher than that of CIPp and CIPm. The cell proliferation and vigor of CIPm was the weakest （p〈0.01）. The results demonstrated that tumor cell lines deriving from different tissues have significantly biological characteristics. The cell line of mammary gland tumor CHMp and CHMm have higher sensitivity in the study of mammary gland tumor and metastasis.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2014年第6期444-447,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金(31372492)
关键词
犬乳腺肿瘤
细胞系
p53因子
生物学特性
canine breast tumor
cell line
p53 factors
biological characteristics
