摘要
目的:研究蛇毒三肽pENW对体外过氧化氢诱导人脐静脉内皮细胞损伤的保护作用。方法:用体外培养的人脐静脉内皮细胞株传代后进行实验,H2O2(300μmol/L,12h)模拟氧化损伤模型;药物处理组分别加入不同剂量的蛇毒三肽pENW(10-4mol/L,10-5mol/L,10-6mol/L)以及阳性对照药依达拉奉(10-5mol/L);用噻唑蓝(MTT)比色法检测细胞活力;比色法检测细胞培养上清液中乳酸脱氢酶(LDH)的漏出率,细胞中超氧化物歧化酶(SOD)的活性和谷胱甘肽(GSH-Px)的含量;Annexin V-FITC/PI双染法流式细胞仪检测细胞凋亡。结果:蛇毒三肽pENW(10-4mol/L,10-5mol/L)能够显著抑制H2O2对人脐静脉内皮细胞的氧化损伤作用,降低H2O2诱导人脐静脉内皮细胞的凋亡,降低LDH漏出率,提高人脐静脉内皮细胞中SOD、GSH-Px活性。结论:蛇毒三肽pENW可降低过氧化氢对人脐静脉内皮细胞的氧化损伤作用,其保护作用可能与抗氧化及抑制细胞凋亡有关。
Objective ： To investigate the protective effect of pENW on the injury of human umbilical vein endothelial cells（ HUVECs） induced by hydrogen peroxide in vitro and to explore its underlying mechanism. Methods： The HUVECs line was cultured in vitro for experiment. HUVECs were treated with pENW（ 10-4mol /L,10-5mol /L,10-6mol /L） and edaravone（ 10-5mol /L）,then induced with H2O2（ 300 μmol/L,12h）. The cell viability was evaluated by MTT assay. The superoxide dismutase（ SOD） activities,the glutathione（ GSH） contents and llactate dehydrogenase（ LDH） were measured by commercially available kits spectrophotometrically. The apoptosis of HUVECs was detected with Annexin V-FITC /PI staining by flow cytometer. Results： Compared with normal control group,H2O2can obviously damage vascular endothelial cells,which could be protected by pENW. pENW also could decrease the apoptosis of HUVECs and leakage of LDH as well as increasing the content of GSH and SOD activity. Conclusion： The results showed that pENW could decrease the damage of HUVECs induced by H2O2,which may relate to anti-oxidation and inhibiting the apoptosis.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2014年第2期43-46,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
河南中医学院博士科研基金(BSJJ2012-01)支持
