摘要
目的利用小干扰RNA技术沉默Bmi-1基因的表达,探讨其对人鼻咽癌CNE-1生物学行为的影响。方法设计并化学合成了针对Bmi-1的小干扰RNA,转染具有高转移性的CNE-1细胞,利用qRT-PCR和Western blot分别检测Bmi-1基因mRNA和蛋白的表达水平。体外通过Transwell小室、Matrigel胶模型﹑流式细胞术,观察Bmi-1被沉默后对鼻咽癌细胞株CNE-1生物学行为的影响。结果 qRT-PCR和Western结果显示,Bmi-1-siRNA转染组Bmi-1基因的表达水平被有效抑制。与对照组相比,Bmi-1-siRNA转染组的细胞侵袭迁移力明显下降,细胞被阻滞在S期。结论抑制Bmi-1基因的表达可有效降低CNE-1细胞侵袭迁移的能力。
Aim To investigate the effect of small inter-ference RNA-mediated silcencing of the Bmi-1 gene on cell invasion and metastasis in human nasopharyngeal carcinoma cell line CNE-1 . Methods Chemically syn-thesized siRNA targeting the Bmi-1 gene was transfect-ed into CNE-1 cells, which had high invasive and metastatic potential. The expression of Bmi-1 mRNA and protein were detected by quantative Real-time PCR and Western blot, respectively. The effects of Bmi-1 knockdown on CNE-1 cells migration and invasion were analysied by Transwell migration assay and Matrigel in-vasion assay. Results Transfected with Bmi-1 siRNA significantly down-regulated the expression of Bmi-1 mRNA and protein as compared with the control group. CNE-1 cells transfected with Bmi-1 siRNA had lower levels of invasion and migration capacity than cells in the control group. Conclusion SiRNA-media-ted silencing of the Bmi-1 gene could significantly inhibit cell migration and invasion in human nasopharyn-geal carcinoma cell line CNE-1 .
出处
《中国药理学通报》
CAS
CSCD
北大核心
2014年第6期833-837,共5页
Chinese Pharmacological Bulletin
基金
教育部高等学校博士学科点专项科研基金(No 20125503110012)
教育部高等学校博士学科点新教师基金项目(No 20115503120007)
重庆市自然科学基金项目(No cstc2011jjA10035)
关键词
BMI-1
RNA干扰
侵袭
迁移
周期
增殖
凋亡
Bmi-1
RNA interference
invasion
migration
cycle
proliferation
apoptosis
