摘要
目的探讨黄芩苷对人皮肤鳞状细胞癌A431细胞增殖及迁移能力的影响及其机制。方法 50 mg·L-1黄芩苷孵育A431细胞,Western blot检测细胞中cofilin-1蛋白的表达水平;设计合成cofilin-1特异性siRNA并转染A431细胞,使用CCK8法检测细胞增殖活性,细胞划痕试验检测细胞的迁移能力。结果 Western blot结果显示,与对照组相比,细胞cofilin-1蛋白表达下降了49.3%;单独黄芩苷处理和cofilin-1-siRNA沉默均可抑制A431细胞生长与迁移,合并cofilin-1-siRNA则明显增强黄芩苷的作用效能。结论黄芩苷可有效降低肿瘤细胞的增殖活性和迁移能力,cofilin-1基因可能成为增强药物作用的调控靶点之一。
Aim To investigate the effect of baicalin on cell proliferation and cell migration in human skin SCC A431 cell line. Methods The A431 cells were incubated with 50 mg·L-1 baicalin. The protein level of cofilin-1 was assayed by Western blot. Cofilin-1 specific siRNA fragment was designed , synthesized and transfected into A431 cells. The proliferative activity and migration ability of cells were assessed by CCK8 assay and scratch wound healing assay separately. ResultsWestern blot results showed that baicalin treatment in-hibited the cofilin-1 protein expression to 49.3% com-pared with the control group. Single baicalin treatment and cofilin-1 silencing could drease the A431 cell growth and migration. And cofilin-1 silencing significantly enhanced the efficacy of baicalin. Conclusions Baicalin could significantly inhibit the tumor cell＇s growth and migration in the A431 cell line. And cofi-lin-1 might become the potential target gene to enhance the effect of anticancer drugs.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2014年第6期821-825,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 30876232)
江苏省自然科学基金资助项目(No BK2012170)
江苏省卫生厅中医药科技项目(No LZ11095)
