摘要
目的探讨类风湿关节患者血清中IL-27、炎症指标和疾病活动度的关系。方法选择97例类风湿关节炎患者和43例健康对照者,通过免疫比浊法检测血清中的C反应蛋白、类风湿因子;ELISA试剂盒检测血清IL-27和抗环瓜氨酸抗体(CCP)水平;魏氏法测定红细胞沉降率;用Mann-Whitney秩和检验比较RA患者和健康对照者血清中IL-27的差别,并对服药前后的患者体内的IL-27水平进行比较;对各指标间的关系采取Spearman相关性分析。结果 RA患者的IL-27血清学水平显著高于所有正常对照(P<0.001),并且IL-27的水平与RA的疾病活动度相关(r=0.299,P=0.039),经过免疫抑制剂治疗后RA患者血清IL-27水平显著下降(P<0.001)。结论 IL-27与CCP、ESR、CRP等指标相关性不大,但与疾病的活动度(DS28)密切相关,因此,IL-27参与了RA的疾病过程,将是一个较好的指示RA疾病活动度的早期指标。
In order to explore the relationship between the serum IL-27 and the inflammatory markers as well as disease activity in rheumatoid arthritis （RA）, 97 cases of RA patients and 43 healthy controls were recruited. The serum levels of C-reactive protein, rheumatoid factor （RF）, IL-27, anti-cyclic eitrullinated antibody （CCP）, erythroeyte sedimentation rate（ESR） were determined by immunoturbidimetry, ELISA assay and Westergren method respectively. Then Spearman＇s rank correlation test was used to assess the correlations of serum IL-27 concentrations with RF, anti-CCP, CRP and ESR levels. We found that the level of serum IL-27 was significantly higher in RA patients than that in control subjects. Furthermore, serum IL-27 concentration exhibited a positive and significant correlation with DAS28 score in all RA patients. The results suggested that IL-27 is involved in the RA disease, and might be a good indicator for early RA disease activity index.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2014年第6期536-538,共3页
Immunological Journal
基金
国家临床重点专科建设项目经费资助[财社2010]305号
重庆市卫生局课题面上项目(2011-2-120)
关键词
IL-27
类风湿关节炎
炎症
免疫反应
Serum IL-27
Rheumatoid arthritis
Inflammation
Immune response
