摘要
目的研究脂多糖刺激后对微小RNA(miR-155)诱导B淋巴细胞肿瘤样变的影响。方法实时定量聚合酶链式反应(qRT-PCR)检测脂多糖刺激后0 h、4 h、12 h、24 h、48 h B淋巴母细胞中miR-155的表达量,同时检测转录因子PU1mRNA和蛋白水平的表达,流式细胞技术检测B细胞表面分子CD10表达变化。结果脂多糖刺激B淋巴细胞后,随着时间的增加,miR-155表达呈递增趋势,增至正常水平3.6倍,刺激前后B淋巴母细胞miR-155表达差异有统计学意义(t=6.76,P<0.05)。而转录因子PU1表达水平呈降低趋势,刺激前后B淋巴母细胞PU1表达差异有统计学意义(t=4.22,P<0.05)。脂多糖刺激B淋巴细胞24 h后CD10数量由(32.10±2.82)%上升到(80.60±3.14)%,差异有统计学意义(t=2.13,P<0.05),B淋巴细胞增殖能力增强,细胞克隆数增加近4倍,差异有统计学意义(t=2.56,P<0.05)。结论 miR-155通过转录因子PU1间接调控CD10分子的表达,诱导B细胞的肿瘤样变,推断miR-155在B淋巴细胞的成瘤过程中发挥重要作用。
Objective To investigate the effect of lipopolysaccharide （LPS） stimulation on mir-155 regulating B lym-phoma tumor-like lesions. Methods The miR-155 expression, PU1mRNA and protein levels were detected by qRT-PCR at 0 hour ,4 hours,12 hours,24 hours and 48 hours after LPS stimulation. The CD10 expression were analyzed by flow cy-tometry. Results The miR-155 expression level in the B cells was increased with the increasing time after LPS stimula-tion and increased to 3.6 times the normal level. The difference of B lymphoblastoid cell miR-155 expression before and after stimulation was statistical significantly as well as the expression of PU1 （t=6.76, 4.22, P＆lt;0.05）. The level of CD10 at 24 hours after LPS stimulation was increased from（32.10＆#177;2.82）% to （80.60＆#177;3.14）%, the difference was statistical sig-nificantly （t=2.13,P＆lt;0.05）. LPS stimulation enhanced the proliferation capacity of B Lymphoma and cell clone number was increased nearly four times, the difference was statistical significantly （t=2.56,P＆lt;0.05）. Conclusion The miR-155 regulates the expression of CD10 molecules indirectly through transcription factors PU1 and induces B lymphoma tumor-like lesions. The miR-155 plays an important regulator in the development of B lymphoma.
出处
《全科医学临床与教育》
2014年第2期126-130,共5页
Clinical Education of General Practice
