摘要
将奥尼罗非鱼(Oreochromis niloticus×O.areus)幼鱼暴露于不同质量浓度(0、2.5 mg·L-1、5 mg·L-1、10 mg·L-1和20 mg·L-1)的氨氮溶液中,于第0、第12、第24、第48和第72小时测定其非特异性免疫相关指标。结果表明,试验幼鱼经氨氮胁迫后各试验组血清溶菌酶活力随着胁迫时间的延长下降显著(P<0.05)。氨氮胁迫明显影响鱼肝脏总抗氧化能力(T-AOC)及相关抗氧化酶活力。与对照组相比,随胁迫时间增加各试验组肝脏TAOC及总超氧化物歧化酶(T-SOD)活力均先下降后上升(P<0.05)。过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活力变化还与胁迫溶液浓度相关。低浓度组(2.5 mg·L-1和5 mg·L-1),CAT活力在胁迫24 h后显著下降(P<0.05);高浓度组(10 mg·L-1和20 mg·L-1),CAT活力却出现先升高后降低的变化。除最高浓度组,各试验组GSH-Px活力表现为诱导效应(P<0.05)。试验条件下氨氮胁迫对罗非鱼非特异性免疫产生明显影响,且随浓度增加及时间延长影响程度加大。
The juveniles of tilapia（Oreochromis niloticus ＆#215; O. Areus）were exposed to ammonia-N（0,2. 5 mg·L - 1 ,5 mg·L - 1 , 10 mg·L - 1 and 20 mg·L - 1 ）for 0,12 h,24 h,48 h and 72 h to evaluate the effect of ammonia-N stress on their non-specific immunity. Results show that the activity of serum lysozyme decreased significantly with extension of stress time（P ﹤ 0. 05）. The total antioxidant capacity（T-AOC）and activity of antioxidase in liver were significantly affected. The activity of T-AOC and total superox-ide dismutase（T-SOD）of fish exposed to ammonia-N were initially decreasing then increasing（ P ﹤ 0. 05）. Activities of catalase （CAT）and glutathione peroxidase（GSH-Px）were correlated with concentrations of ammonia-N. Fish exposed to lower concentrations（2. 5 mg·L - 1 ,5 mg·L - 1 ）showed decreasing CAT activity within 24 h（P ﹤ 0. 05）,while those exposed to higher concentrations（10 mg·L - 1 ,20 mg·L - 1 ）showed initially increasing then decreasing activity of CAT. Except for the highest concentration groups,fish exposed to ammonia-N showed induction activity of GSH-Px（P ﹤ 0. 05）. Under the experimental conditions,non-specific immunity of tilapia was affected by ammonia-N stress,and the impact was increasing with increasing concentration and extension of time.
出处
《南方水产科学》
CAS
CSCD
北大核心
2014年第3期47-52,共6页
South China Fisheries Science
基金
广东省自然科学基金项目(S2013010013693
S2011010003451)
嘉应学院自然科学研究重点项目(2011KJZ05)
广东省优秀青年教师计划项目(Yq2013152)
关键词
罗非鱼
氨氮胁迫
非特异性免疫
Oreochromis niloticus×O. areus
ammonia-N stress
non-specific immunity
