摘要
目的:探讨微囊藻毒素-LR(MC-LR)对模式生物秀丽隐杆线虫精子形成的毒性作用。方法:L4期him-5雄虫分别暴露于0.1、1、10和100μg/L的MC-LR中,染毒48 h。通过测定精细胞大小和体外活化率分别观察MC-LR对精子竞争力和活化能力的影响,并利用实时荧光定量PCR测定相关基因表达水平,用杂交试验验证MC-LR对雄虫生育力的影响。结果:暴露48 h后,100μg/L组秀丽隐杆线虫精细胞与对照组相比周长和面积均显著减小(P均<0.01);10和100μg/L染毒组精细胞体外活化率显著下降(P均<0.01);spe-10和spe-15基因在染毒组出现了表达水平下调的现象;各染毒组后代数目与对照组相比差异均无统计意义(P均>0.05)。结论:MC-LR对秀丽隐杆线虫精子形成过程具有一定的毒性作用。
OBJECTIVE：To study the toxicity of spermiogenesis caused by MC-LR exposure. METHODS：L4 him-5 strain males were exposed to MC-LR at 0.1,1,10 and 100 μg/L for 48 h. The ability of spermatid activation was measured by testing its rates in vitro. Spermatid size was measured to assess its competitiveness. Expression levels of the related genes were evaluated by qRT-PCR. Male fertility was measured by brood sizes of him-5 males. RESULTS：After 48-hour exposure,the 100μg/L group showed reduced spermatid size （P〉0.01）. Exposures to 10 and 100 μg/L reduced the rates of spermatid activation （P〈0.01）. Expression levels of spe-10 and spe-15 were significantly decreased after exposure. No significant decreases in brood sizes were observed. CONCLUSION：MC-LR may impair the process of spermiogenesis.
出处
《癌变·畸变·突变》
CAS
CSCD
2014年第2期140-143,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金项目(81273123)
关键词
秀丽隐杆线虫
精子形成
生殖毒性
Caenorhabditis elegans
microcystins-LR
spermiogenesis
reproductive toxicity
