摘要
研究用超临界CO2从发酵菌丝中萃取霉酚酸（MPA）的工艺，采用响应面．小星点设计，考察了夹带剂乙醇浓度（55％～95％）、萃取时间（30～150min）、萃取压力（20～40MPa）、萃取温度（40～80℃）对MPA萃取回收率（RR）和提取物中MPA含量（GMPA）的影响。响应面分析表明，R尺与CMPA均与该4因素符合二次模型，萃取时间的一次方、二次方和萃取压力均高度影响艘，而乙醇浓度的一次方和二次方、萃取时间的二次方以及乙醇浓度和萃取温度的交互作用都对GMPA有极显著影响。根据所得二次响应面模型预测最佳工艺条件，综合考虑艘和GMPA，确定MPA的超临界CO，萃取工艺为：以95％乙醇为夹带剂、萃取压力20MPa、萃取温度40℃、萃取150min。采用该工艺进行3次验证试验，艘为95．58％±1．12％，GMPA为70．54％±1．04％，与预测值96．16％和70．53％相符。
The supercritical CO2 extraction process of mycophenolic acid （MPA） was studied by response surface method. A 4-factor, 5-level small central composite design was used to optimize the four independent variables, ethanol content in modifier （55%-95%）, extraction time （30-150min）, extraction pressure （20-40MPa）, and extraction temperature （40-80℃）, with recovery rate of MPA （RR） and MPA contents in the extracts （CMPA） as dependent variables. The quadratic polynomial formulae were obtained by response surface analysis to describe the relationships between RR and the four parameters and between Cmn and the four parameters. The linear and quadratic terms of time and the linear term of pressure influenced RR significantly. Whereas, the linear and quadratic terms of ethanol content, the quadratic term of time, and the interaction between ethanol content and temperature had significant effects on CMPA. Taking RR and CMPA into account simultaneously, the optimal extraction conditions was determined, according to the second-order polynomial models, to be 20MPa, 40℃, 150 min, and with 95% ethanol as modifier. Three verification tests were completed with this optimized technique, and the RR and CMPA were 95.58%±1.12% and 70.54%±1.04%, respectively, coinciding with the predicted values, 96.16% and 70.53%, respectively.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2014年第6期422-428,共7页
Chinese Journal of Antibiotics
基金
"重大新药创制"科技重大专项"原料药的技术升级和再创新"(No.2011ZX09203-001-11)
关键词
霉酚酸
超临界CO2萃取
响应面分析
小星点设计
Mycophenolic acid
Supercritical CO2 extraction
Response surface methodology
Small central composite design
