摘要
目的 探讨葡聚糖修饰的超顺磁性氧化铁（super-paramagnetic iron oxide,SPIO）与多聚赖氨酸（polylysine,PLL）纳米粒的制备及其结构验证和空间结构表征。方法 在共沉淀法制备葡聚糖修饰的SPIO基础上,加入PLL制备出一种新的SPIO-PLL纳米粒,利用粒度电位分析仪、透射电镜（transmission electron microscopy,TEM）对SPIO-PLL的粒径、电位、形貌学进行检测;高效液相凝胶色谱法（gel permeation chromatography,GPC）、红外光谱（fourier transform infrared spectroscopy,FTIR）及核磁共振氢谱法（1HNMR）对SPIOPLL连接成功与否进行验证;高精度的原子力显微镜（atomic force microscope,AFM）对SPIO-PLL的结构、形态及连接状态进行空间结构表征。结果 粒度电位分析和TEM结果显示SPIO-PLL的平均粒径为7.37 nm,平均电位为13.6 mV;GPC、FTIR及1HNMR结果显示SPIO及PLL之间已经成功连接;高精度AFM清楚显示出SPIO与PLL之间以亚胺键形式稳定相连。 结论 成功制备出一种性质稳定、粒径较小的新型SPIO-PLL纳米粒,这将对随后的肿瘤特异性探针制备奠定一定的基础。
Objective To explore the preparation,structure validation and space form characterization of super-paramagntic iron oxide-polylysine （SPIO-PLL） nanoparticles,which was coated by Dextran then modified by polylysine （PLL）.Methods A novel type of nanoparticles was prepared on the basis of SPIO obtained by coprecipitation,and then superficial modified with PLL.The hydrodynamic size,zeta potential and shape of products were detected by particle potential analyzer and TEM.While the connection of products was characterized by gel permeation chromatography （GPC）,fourier transform infrared spectropy （FTIR） and 1HNMR.Furthermore,high-resolution atomic force microscopy （AFM） was utilized to characterize the molecular structure of products and the interaction between SPIO coated by Dextran and PLL.Results The hydrodynamic size,zeta potential and TEM results showed the average size and zeta potential of products was 7.37 nm and 13.6 mV,respectively.The successful connection of SPIO and PLL was proven by GPC,FTIR and 1HNMR.Moreover,AFM findings clearly revealed the successful connection of SPIO and PLL in form of imine linkage.Conclusions A novel type of good quality SPIO-PLL nanoparticles whose average size was 7.37 nm was prepared and it may lay a certain foundation for specific tumor probe preparation.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2014年第3期309-314,327,共7页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金(81171366)~~
