摘要
应用miRtour在线分析工具对巨桉的EST序列和GSS序列进行分析,预测巨桉的miRNA序列,应用psrobot预测miRNA的靶基因。结果发现205条miRNA前体序列和属于62个不同家族的170条成熟的miRNA序列,最大的miRNA家族为miR399家族,有17个成员;miRNA 5′段碱基存在明显的碱基偏倚,尿嘧啶出现频率高达40.6%;147个miRNA预测到了靶基因,共计预测到巨桉蛋白基因中有967个受到miRNA的调节,同时发现1个miRNA可以调控多个靶基因,同一蛋白质受多个miRNA调控的现象。
MicroRNA of Eucalyptus grandis was predicted using EST and GSS by miRtour, whereas miRNA-targeted mRNAs was predicted by Psrobot. 205 precursor sequences and 170 miRNAs belonging to 62 different miRNA familes were found. The largest miRNA family of 17 members is miR399. The uracil nucleotide is dominant in the first position of 5＇ mature miRNAs, which is up to 40.6%. 147 miRNA has potential miRNA targets meanwhile 967 protein genes may be regulated by miRNA. In addition, it found that miRNA can regulate multiple target genes while one protein can be regulated by some miRNAs.
出处
《热带作物学报》
CSCD
北大核心
2014年第6期1108-1116,共9页
Chinese Journal of Tropical Crops
