摘要
目的:探讨新疆哈萨克族食管癌患者ALDH1L1基因甲基化状态及其与食管癌发生及预后之间的关系。方法:采用联合亚硫酸氢钠限制性内切酶分析(COBRA)法检测28例哈萨克族食管癌及癌旁组织标本中ALDH1L1基因启动子区甲基化状况,建立Cox回归模型分析临床病理指标、ALDH1L1基因甲基化水平等因素与预后的关系。结果:28例哈族食管癌患者癌组织和癌旁正常组织的ALDH1L1基因启动子甲基化率分别为71.43%和39.28%,差异有统计学意义(P<0.05)。预后因素分析表明肿瘤浸润程度、TNM分期、淋巴结转移、癌组织ALDH1L1基因甲基化水平是影响哈萨克族食管癌预后的独立危险因素。癌组织ALDH1L1基因无甲基化患者术后生存时间长于完全甲基化患者(P<0.05)。结论:癌组织ALDH1L1基因启动子区甲基化状态与新疆哈萨克族食管癌的发生及预后有关。
OBJECTIVE：To explore the relationship between the hypermethylation status of ALDH1L1 gene and the occurrence and prognosis of esophageal carcinoma（ EC） in Kazakh ethnic groups in Xinjiang.METHODS：The hypermethylation of ALDH1L1 promoter region was evaluated by using combined bisulfite restriction analysis （COBRA） in 28 cases of esophageal cancer tissues from Kazakh patients.Cox model was established to analyze the possible factors influencing the prognosis of EC,including clinicopathological parameters and hypermethylation levels of ALDH1L1 promoter region. RESULTS：The methylation rates of ALDH1L1 promoter region in esophageal cancer tissues and adjacent tissues were 71.43%and 39.28%,respectively. Significant difference were found in the methylation rates of ALDH1L1 promoter region in esophageal cancer and adjacent tissues （P〈0.05）. Multivariate prognostic analysis showed that the infiltration,TNM stage,lymph node metastasis and hypermethylation levels of ALDH1L1 were independent risk factors. The postoperative survival time of EC patients in Kazakh ethnic with ALDH1L1 gene unmethylation in cancer was significantly longer than those with ALDH1L1 gene methylation （P〈0.05）. CONCLUSION：Aberrant methylation of ALDH1L1 promoter region may play an important role in the pathogenesis and prognosis of EC in Kazakh ethnic groups.
出处
《癌变·畸变·突变》
CAS
CSCD
2014年第1期35-39,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助项目(81060240)
