摘要
目的 研究星形胶质细胞对神经生长因子 (NGF)的表达及白细胞介素 - 1α(IL- 1α)与白细胞介素- 1β(IL- 1β)对星形胶质细胞表达 NGF的影响。方法 取新生 SD大鼠大脑皮质 ,纯化培养星形胶质细胞 ,设空白组、对照组和实验组三组 ,实验组中分别加入 2 5、5 0和 10 0 U/ m l的 IL- 1α或 IL- 1β,分别培养 2 4、48及 72小时 ,取培养上清液 ,用间接酶联免疫吸附法测定星形胶质细胞培养上清液中的 NGF分泌量。结果 传代培养的星形胶质细胞能够分泌 NGF,不同浓度的 IL- 1α及 IL- 1β作用不同时间后均不同程度地促进星形胶质细胞分泌 NGF,其效应IL- 1β较 IL- 1α为强 ,且单位时间内的效应以 5 0 U/ m l组作用 2 4小时最强。结论 星形胶质细胞自身能够表达NGF,而 IL- 1α及 IL- 1β可不同程度地促进星形胶质细胞对
Objective To study the nerve growth factor (NGF) expression and the influence of IL-1α or IL-1β on NGF secretion in newborn rat astrocytes. Methods Astrocytes obtained from the brain cortex of newborn rats were cultured and purified, and they were divided into three groups, experimental, control and blank groups. IL-1α or IL-1β were added into the experimental group with 25, 50 and 100U/ml, each group was cultured for 24, 48 or 72 hours, and then the NGF contents in cultured astrocytes suspension media were measured by a two-cite enzymelinked immunoserbent assay (ELISA). Results Astrocytes could secret NGF by themselves and each concentration of IL-1α or IL-1β media at any testing time could enhance NGF secreting in newborn rat astrocytes in certain degrees. The effects of IL-1β were stronger than IL-1α, the strongest effect in the unit time was observed in IL-1β with 50U/ml for 24 hours. Conclusion Astrocytes can express NGF, and IL-1α or IL-1β can enhance the NGF expression in newborn rat astrocytes.
出处
《中国修复重建外科杂志》
CAS
CSCD
2001年第2期106-108,共3页
Chinese Journal of Reparative and Reconstructive Surgery
