摘要
目的 探索pRB和E2 F1mRNA在细胞周期中的作用及人肺神经内分泌肿瘤的发生机制 ;以及与肿瘤侵袭性和预后的相关性。方法 分别采用免疫组化和分子原位杂交技术对 78例人肺神经内分泌肿瘤的石蜡切片标本中的pRB和E2 F1mRNA表达进行检测 ,通过统计学方法研究其表达与肿瘤分期、淋巴结转移及预后的相互关系。结果 pRB在典型类癌和非典型类癌中的阳性表达率为 87 5 % (14/16 ) ,而在大细胞神经内分泌癌和小细胞肺癌中则不表达 (P <0 0 0 1) ;E2 F1mRNA在人肺神经内分泌肿瘤中的阳性表达率为 87 2 % (6 8/78) ,二者的表达强度及相互关系与肿瘤的恶性程度、淋巴结转移及预后相关 (P <0 0 5 )。结论 pRB丢失是人肺神经内分泌肿瘤发生恶性变的关键。E2 F1过度表达是人肺神经内分泌肿瘤发生的一个重要因素。
Objective To investigate the role pRB and E 2F 1 play in the cell cycle and the oncogenesis of human nemoendocrine lung fumor and the correlation between their expression and the invasiveness and prognosis of human neuroendocrine lung tumor. Methods Immunohistochemistry and in situ hybridization were used to detect the expression of pRB and E 2F 1 mRNA in the paraffin embedded tissues of 78 cases of human neuroendocrine lung tumor. Statistics was used to analyze the data. Results The positive expression rate of pRB in both typical and atypical carcinoid was 87.5%(14/16). However, pRB was not expressed in large cell neuroendocrine carcinoma and small cell lung cancer ( P <0.001). The positive rate of E 2F 1 mRNA expression in human neuroendocrine lung tumor was 87.2% (68/78). The strength of expression of pRB and E 2F 1 was obviously correlated with the tumor stage, lymph node metastasis and prognosis ( P <0.05). Conclusion The loss of pRB and E 2F 1 overexpression may play a key role in the development of human neuroendocrine lung tumors.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2001年第4期219-221,共3页
National Medical Journal of China
