摘要
目的研究0.05g·L^(-1)～3.5g·1^(-1)浓度苦参碱对人肝癌细胞株HepG2的增殖抑制及其剂量与抑制方式的关系,为进一步研究苦参碱抑制HepG2细胞增殖的机制打下基础;为临床应用苦参碱治疗肿瘤奠定基础。方法苦参碱处理细胞后,MTT法、细胞计数法、H^3-TdR掺入法测定HetG2细胞存活率、细胞生长曲线、DNA合成率的变化;台盼蓝拒染法和细胞外液LDH活性测定观察细胞膜的完整性。结果苦参碱浓度小于0.2g·L^1时不能抑制HepG2的增殖,细胞存活率大于80％;0.7g·L^(-1)～0.8g·L^1时,HepG2对药物中度敏感,细胞存活率为30％～50％:大于1.0g·L^1时为抗HepG2的敏感药物浓度,细胞存活率小于30％;苦参碱大于3.0g·L^(-1)时,细胞膜完整性破坏,以坏死细胞为主。而苦参碱对大鼠肝细胞影响很小,达1.5g·L^(-1)时,细胞存活率仍为72.4％。结论一定浓度苦参碱可抑制HepG2细胞增殖并且抑制作用呈时间和剂量依赖性。0.8g·L^(-1)可作为诱导HepG2细胞分化的作用浓度;1.5g·L^(-1)～2.5g·L^(-1)可作为诱导HepG2凋亡的作用浓度;苦参碱大于3.0g·L^(-1)时主要引起HepG2坏死。
AIM To study 0.05-3.5 g.L^1 matrine inhibiting proliferation of human hepatoma cell HepG2 and the relation between its dosage and inhibiting style to lay a foundation for further study on its mechanism of action and for clinical application for tumor treatment. METHODS After cells were treated by matrine, the cell survival rate, the inhibited DNA synthetic rate and the cell number change were detected with MTT assay, H^3-TdR incorporation methA and cell counting methA. The cell necrosis was observed with Trypan blue staining methA. RESULTS When the drug concentration was lower than 0.2 g·L^(-1), matrine could not inhibit proliferation of HepG2, the cell survival rate was more than 80%;when the concentration was 0.7-0.8 g·L^(-1). HepG2 was moderately sensitive to matrine, the cell survival rate was 30%-50%. When the concentration was higher than 1.0g.L^(-1), HepG2 was sensitive to matrine, the cell survival rate was less than 30%. When the concentration was higher than 3.0 g·L^1,cell membrane integrity was damaged with an increase of necrosis. However, effect of matrine on normal rat liver cells was minor, when matrine was 1.5 g·L^(-1), the cell survival rate remained at 72.4%. CONCLUSION Matrine's inhibiting effect on the HepG2 depends on its working time and dosage. The concentrations of 0.8 g·L^(-1) and 1.5 g·L^(-1), were chosen respectively in our investigation as the working concentration to induce the cell differentiation and apoptosis; matrine of higher than 3.0 g·L^(-1) caused mainly necrosis of HepG2.
出处
《世界华人消化杂志》
CAS
2001年第2期185-189,共5页
World Chinese Journal of Digestology
关键词
苦参碱
药理学
剂量
肿瘤细胞
药物作用
matrine/pharmacology
matrine/administration & dosage
liver neoplasms/pathology
tumor cells, cultured/drug effects
