摘要
漆酶是一种生物蛋白酶,容易变性失活且有不可重复使用的特性,限制了漆酶的应用和发展。因此,如何提高漆酶的稳定性和重复使用性尤为重要。本文采用交叉偶联技术,将漆酶固定在具有核壳结构的磁性纳米粒子Fe3O4/SiO2上,探讨了密闭条件下热处理对固定化漆酶热稳定性的影响,及固定化漆酶降解不同浓度普施安的活性。结果表明:Fe3O4\SiO2纳米粒子担载的漆酶经70℃热处理后,仍有70%的活性,表现出良好的热稳定性;固定化漆酶对不同初始浓度普施安的脱色反应,符合一级反应。
Laccase is a kind of biological protease, however, the easy denaturation and difficult separation for repeat use limit the application and development of laccase. So, how to improve the stability of laccase and repeatability is very important. In present study, laccase was immobilized on magnetic Fe3O4/SiO2 nanoparticles through cross-coupling method to further discuss the thermal stability of immobilized laccase after being heated in an airtight container and the degradation of procion（PR） with different initial concentrations. The results show that the immobilized laccase after being treated at 70℃ still keep 70% of the initial activity, showing good thermal stability. In the meantime, the decolorization of Procion with different initial concentrations fits the first-order reaction.
出处
《化学工程师》
CAS
2014年第5期14-16,34,共4页
Chemical Engineer
基金
黑龙江省自然科学基金(B201011)
