摘要
用焦锑酸钾沉淀法研究了水稻红莲 -粤泰不育系及其保持系花药发育过程中 Ca2 + 的分布变化。结果表明 :二核期前后 ,可育花粉和败育花粉表面均积累较多 Ca2 + 的沉淀颗粒 ;可育花粉内充满浓密细胞质和淀粉粒 ,无 Ca2 + 沉淀颗粒分布 ;败育花粉在其细胞质的液泡中积累大量 Ca2 + 沉淀颗粒 ,胞质中出现液泡化网状结构 ,并逐步解体成空泡。保持系药隔维管束及薄壁细胞中几乎无 Ca2 +沉淀颗粒分布 ;而不育系药隔维管束鞘及其周边细胞、导管与筛管的内壁和液泡中均有较多 Ca2 +沉淀颗粒分布 ,薄壁细胞的核分布着较少 Ca2 + 沉淀颗粒。并对不育系花粉内和药隔组织细胞中 Ca2 + 大量积累的异常现象作了深入的探讨。
Potassium antimonate was used to locate Ca 2+ in fertile and sterile anthers of Honglian Yuetai cytoplasmic male sterile rice( Oryza sativa L.). During the development of fertile and sterile anthers, abundant calcium precipitates accumulated on the outer surface of pollen walls at the binucleate stage while starch grains accumulated in pollen. There were no C 2+ a precipitates in the cytoplasm of fertile pollen grains. However, a great quantity of calcium precipitates accumulated in central vacuole of abortive pollen. There was not distribution of calcium precipitates in connective tissue of fertile anthers. Alternatively, in connective tissue of sterile anthers, abundant calcium precipitates located in vacuole and cytoplasm of vascular bundle sheath cells and its peripheral cells, vessels and sieve tubes, but few calcium precipitates in nuclei of parenchymatous cells. The changes of antimonate induced calcium precipitates in the pollen and connective tissue of cytoplasmic male sterile rice indicate that the anomalies in the distribution of calcium accumulation correlate with pollen abortion.
出处
《作物学报》
CAS
CSCD
北大核心
2001年第2期230-235,共6页
Acta Agronomica Sinica
基金
国家自然科学基金!资助项目 (编号 :3 9770 4 55)&&
