摘要
将鸭瘟粗提病毒经SephadexG2 0 0柱层析纯化后作为包被抗原 ,以健康鸭IgG提纯后免疫羊 ,制备羊抗鸭IgG ,并用过碘酸钠法进行辣根过氧化物酶标记 ,建立了检测鸭瘟病毒抗体的间接酶联免疫吸附试验 (ELISA)。经对不同血清样品的检测 ,表明此方法特异性高 ,重复性好 ,操作简便 ,适于大批鸭群抗体水平监测和流行病学调查 ,为合理免疫和科学预防提供了技术手段。
An indirect enzyme_linked immunosorbent assay(ELISA)was established as following method.The crude extraction of plague virus was purified as coating antigen by means of sephadex_G200 chromatography.The goat was immunized by negative antisera from the ducks free from DPV for goat IgG against duck,which was labelled with horseradish peroxidase(HRP)by Nalo 4.Maternal antibodies and adaptive immune antibodies from duckling and the sera sample from the ducks in different clinical immune Qtatus were examined by ELISA.The results showed that the method is high specificity and sensitivity for detection of antibodies against DPV,and can be used in clinical serological surveillance.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2001年第2期140-143,共4页
Chinese Journal of Preventive Veterinary Medicine
