摘要
目的:研究青天葵水提取液、醇提取液和水醇提取液对金黄色葡萄球菌、大肠杆菌、白色念珠菌、伤寒沙门菌、绿脓杆菌、黑曲霉菌6种菌株的抑菌效果。方法:制备青天葵3种提取液,采用试管两倍稀释法测定3种提取液对金黄色葡萄球菌等6种菌株的最低抑菌浓度(MIC)。结果:青天葵水提取液对伤寒沙门菌的抑菌作用较强(MIC为12.5%),对金黄色葡萄球菌、大肠杆菌、绿脓杆菌和黑曲霉菌的抑菌作用较弱(MIC为50%);醇提取液对伤寒沙门菌和绿脓杆菌都有很强的抑菌作用(MIC为6.25%),对金黄色葡萄球菌也有较强的抑菌活性(MIC为12.5%),对大肠杆菌的抑菌作用较弱(MIC为50%);水醇提取液的抑菌活性与醇提取液相当。结论:青天葵对金黄色葡萄球菌等6种菌株表现出不同程度的抑制活性。
Objective： To study The inhibitory effect of Folium Nerviliae water extract, Alcohol extract, Hydroalcoholic extract to the 6 kinds of bacterial： Staphylococcus aureus, escherichia coli, staphylococcus aureus, salmonella typhosa, pseudomonas aeruginosa and aspergillus niger. Methods： Three kinds of Folium Nerviliae extract were prepared, two times dilution method was used for determinating MIC of three kinds of extract to 6 kinds of bacterial. Results： Folium Nerviliae Water extract showed strong antibacterial activity to salmonella typhosa（MIC was 12.5%）,showed weak antibacterial activity to staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and aspergillus niger（MIC was 50%）, alcohol extract showed strong antibacterial activity to salmonella typhosa and pseudomonas aeruginosa（MIC was 6.25%）, showed relative strong antibacterial activity to staphylococcus aureus（MIC was 12.5%）, showed weak antibacterial activity to escherichia coli（MIC was 50%）, water-alcohol extract＇s antibacterial activity was similar to alcohol extract. Conclusion： Folium Nerviliae shows different degrees of inhibitory activity to 6 strins.
出处
《现代生物医学进展》
CAS
2014年第14期2753-2756,共4页
Progress in Modern Biomedicine
关键词
青天葵
体外抑菌
两倍稀释法
Folium Nerviliae
Antibacterial in vitro
Double dilution method
