摘要
目的探讨酚妥拉明对去甲肾上腺素诱导大鼠心肌肥厚心肌细胞外基质(ECM)重塑的影响及可能机制。方法 24只雄性SD大鼠随机分为对照组、去甲肾上腺素造模组(模型组)和去甲肾上腺素+酚妥拉明组(治疗组)。采用超声心动图观察心脏结构及功能变化,测定胶原容积积分(CVF)、羟脯氨酸、心肌胶原含量,用免疫组织化学法检测心肌组织基质金属蛋白酶2和胶原蛋白Ⅰ的蛋白表达。结果模型组大鼠发生左心室肥厚,其羟脯氨酸含量、CVF值显著高于对照组(P<0.01),基质金属蛋白酶2和胶原蛋白Ⅰ蛋白表达上调(P<0.01)。治疗组心肌肥大明显改善,羟脯氨酸、CVF降低,基质金属蛋白酶2和胶原蛋白Ⅰ蛋白表达下降(P<0.05)。结论酚妥拉明可有效减轻SD大鼠心肌肥厚的发生及ECM重塑;酚妥拉明缓解ECM重塑可能与其降低心肌组织中基质金属蛋白酶2和胶原蛋白Ⅰ的表达有关。
Aim To study the effects of phentolamine on myocardial extracellular matrix of cardiac remodeling induced by norepinephrine in rats. Methods Twenty-four male SD rats were randomly divided into control group, norepinephrine group（ model group） and norepinephrine ＋ phentolamine group（ treatment group）. Left ventricular structure and function among groups of rats were measured by echocardiography. Extracellular matrix remodeling was evaluated by morphological examination,stained with Van-Gieson（ VG）. The content of hydroxyproline in the tissue of myocardium was measured. The protein expression of matrix metalloproteinase-2（ MMP-2） and collagen Ⅰ were examined by immunohistochemical analysis. Results Compared with control group,left ventricular hypertrophy,the hydroxyproline content,collagen volume fraction（ CVF） and protein expression of MMP-2 and collagen Ⅰ was significantly higher in the model group rats（ P〈0. 01）. In treatment group rats,myocardial hypertrophy obviously improved,hydroxyproline,CVF reduced,protein expression of MMP-2 and collagenⅠdecreased（ P〈0. 05）. Conclusion Phentolamine can prevent the cardiac hypertrophy and extracellular matrix remodeling,which was associated with the attenuation of myocardial MMP-2and collagen Ⅰ.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2014年第4期362-366,共5页
Chinese Journal of Arteriosclerosis
