摘要
目的 探讨β3-肾上腺素能受体 (β3- AR)基因 Trp6 4Arg突变与肥胖的关系。方法 采用聚合酶链反应 -限制性酶切片段长度多态性技术检测β3- AR基因型 ;同时检查体质指数 (BMI)等指标 ,观察在 147例肥胖患者和 10 6例健康对照者中 ,β3- AR基因型与 Arg6 4等位基因的频率分布以及不同体质指数的人群中 ,其突变基因的分布情况。结果 肥胖组 Arg6 4等位基因的频率(2 0 .3% )显著高于对照组 (10 .2 % ) (χ2 =10 .14,P =0 .0 0 1) ,突变基因型 Trp6 4Arg、Arg6 4Arg的分布频率 (38.7%和 0 .9% )也高于对照组 (19.0 %和 0 .7% )。在女性人群中 ,肥胖者 Trp6 4Arg基因型及 Arg6 4等位基因频率 (4 0 .6 %和 2 0 .3% )显著高于非肥胖者 (16 .7%和 9.5 % ) ,且差异有显著性 (P <0 .0 1)。结论 β3-肾上腺素能受体基因 Trp6
Objective To investigate the association between the β 3 adrenergic receptor gene (β 3 AR) and obesity. Methods 147 patients with obesity and 106 controls were selected. Genotype of the β 3 AR was determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism methods (RFLP). Body mass index (BMI) was detected. Results The frequency of Arg64 allele of β 3 AR gene in the obesity group (20.3%) was significantly higher than those in the control group (10.2%) (P=0.001). The distribution of Trp64Arg and Arg64Arg genotype in the patient group (38.7% and 0.9%) were both higher than that in the control group (19.0% and 0.7%). In the female subjects, the Trp64Arg mutation and frequency of Arg64 allele of the β 3 AR gene in the obesity group(40.6% and 20.3%) were also higher than those in control (16.7% and 9.5%)(P<0.01). Conclusions These results suggest that the Trp64Arg mutation of the β 3 adrenergic receptor gene may be associated with increased body fat.
出处
《疾病控制杂志》
2001年第1期42-44,共3页
Chinese Journal of Disease Control and Prevention
