摘要
目的探讨肿瘤转移抑制因子Nm23-H1在小细胞肺癌（small cell lung cancer，SCLC）中的表达及其与预后的关系。方法通过免疫组化S-P法检测71例SCLC组织和22例正常肺组织中Nm23-H1蛋白表达情况，根据染色强度和阳性肿瘤细胞数目所占百分比，将其分为高、低表达两组，使用SPSS19．0软件统计分析Nm23-H1的表达与临床病理及预后的关系。结果正常肺组织中Nm23-H1蛋白表达显著高于SCLC组织（90．9％vs57．7％，P〈0．01）；局限期患者Nm23．H1蛋白表达显著高于广泛期患者（66．7％掷39．1％，P〈0．05）。多因素Logistic回归分析显示，Nm23-H1蛋白可作为SCLC是否转移的独立保护因素（OR=0．32，95％C1=0．12—0．90，P〈0．05）。亚细胞定位在正常肺组织与肺癌组织中没有差异，在不同分期之间也没有差异。多因素COX风险回归分析表明只有临床分期为广泛期和年龄I〉60岁是SCLC患者总生存的劣势预后因素（年龄：HR=1．03，95％C1=1．00—1．05，P〈0．05；临床分期：HR：1．85，95％CI=1．03～3．33，P〈0．05）。Nm23．H1蛋白表达及亚细胞定位与SCLC患者生存期都没有显著相关性。结论Nm23-H1在SCLC组织中以细胞质表达为主的低表达，低表达与SCLC的远处转移密切相关，可能是SCLC预后不良的指标之一。
Objective To study the expression of Nm23-H1 and its relationship with prognosis in patients with small cell lung cancer. Methods The expression of Nm23-H1 was detected by immunohisto- chemistry in 71 small cell lung cancer tissue samples and 22 normal lung tissue samples. According to their positive percentage and staining intensity, they were divided into 2 groups：high expression group and low expression group. The correlation of Nm23-H1 expression with clinicopathology and prognosis was performed by SPSS 19.0. Results The positive rate of Nm23-H1 was higher in normal tissue samples than in small cell lung cancer（90.9% vs 57.7% ,P 〈 0.05 ） ;It was higher in limited stage than in extensive stage small cell lung cancer（66.7% vs 39.1% ,P 〈 0.05 ）o Multivariate logistic regression showed the Nm23-H1 expression was an independent protect factor for the metastasis of small cell lung cancer （ OR =0.32,95% CI =0.12 -0. 90 ,P 〈 0.05 ）. The subcellular location of the Nm23-H1 showed no difference between normal lung tissues and cancer tissues, neither between different stages. In the survival analysis, Nm23-H1 positive expression was found to be related with decreased median overall survival than negative expression patients （P 〈 0.05 ）. The stage was also associated with mOS that the patients with limited foei stayed longer than those with metastastic diesease （ HR = 1.85, 95% CI = 1.03 -3.63, P 〈 0.05）. Conclusion Nm23-H1 is midly expressed in SCLC cancer tissues, and mainly in the cytoplasma. The expression plays an important role in the metastasis of SCLC, and may be a predictive factor for the poor outcome of SCLC.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2014年第10期983-986,共4页
Journal of Third Military Medical University
基金
国家自然科学基金面上项目(81272599)~~
