摘要
为研究猪源屎肠球菌的致病力与耐药性及生物被膜形成能力的相关性,本研究分别对9株猪源屎肠球菌进行动物试验、药敏试验和生物被膜形成能力的测定。结果显示有66.67%的菌株引起小鼠肝脏、脾脏出血等病变,并且其中部分菌株导致接种小鼠急性的高死亡率;9株屎肠球菌中KF-QX-1株耐药率最高为100%,其次为ZKXH01(95.24%)、NY01(90.48%)、NY-XY-4(80.95%)、SBLH(76.19%)、HUAX(71.43%)、NY-XY-1(71.43%)和ZHENGY(66.67%),耐药率低于50%的菌株只有JY02(14.29%);生物被膜检测结果显示9株菌中除NY01株外均有不同程度的的被膜形成能力,其中2株菌(JY02和NY-XY-1)生物被膜形成能力相对较强;通过对上述试验结果综合分析显示,肠球菌菌株对受试动物的致病力与其耐药性和生物被膜形成能力之间缺乏明显的相关性,但具有生物被膜形成及耐药两种特性会增强猪源肠球菌的耐药性和传播性,给人类和动物的疾病控制造成更大的威胁。
This study was conducted to determine the correlation between the biofilm formation and antibiotic resistance and pathogenicity in Enterococci faecium isolated from swine. The 9 isolates were tested for pathogenicity through animal experiments and identified for the biofilm formation using microtiter plate method and antibiotic resistance by antimicrobial susceptibility test. The results showed that 66.67% of the Enterococci were manifested comparatively strong virulence, showing the high short-term mortality and mainly with serious hemorrhagic lesions in liver and spleen. Multidrug resistance （≥3 antibiotics） was found in all of E.faecium, which were KF-QX-1 （100%）, ZKXH01 （95.24%）, NY01 （90.48%）, NY-XY-4 （80.95%）, SBLH （76.19%）, HUAX（71.43%）, NY-XY-1 （71.43%）, ZHENGY （66.67%） and JY02 （14.29%）. Biofilm assay indicated that 8 of 9 E.faecium were able to form biofilm in different degrees, of which JY02 and NY-XY-1 developed relatively strong biofilm. In conclusion, these results revealed that no significant correlation was found among antibiotic resistance, biofilm formation and pathogenicity to animal for the E.faecium isolates.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2014年第5期350-353,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家农业科技成果转化项目(30500254)
关键词
肠球菌
致病力
耐药性
生物被膜
相关性
Enterococci
pathogenicity
antibiotic resistance
biofilm formation
correlation
