摘要
采用酶学分析方法研究了温度和pH对驼背鲈3种主要消化酶（蛋白酶、脂肪酶、淀粉酶）活力的影响.结果表明：在设定的温度和pH范围内,驼背鲈消化酶活力均随着温度和pH的升高呈先升后降的趋势.其中,胃蛋白酶的最适温度为40～45℃,肠、幽门盲囊、肝蛋白酶的最适温度均为40℃;胃脂肪酶的最适温度为35℃,幽门盲囊的最适温度为40 ～ 45℃,其他部位均为40 ℃;胃淀粉酶的最适温度为40℃,其他部位均为35℃.胃蛋白酶的最适pH为3.2,肠、幽门盲囊、肝的最适pH均为8.2;胃、肝脂肪酶的最适pH分别为7.2、8.2,其他部位均为6.2;胃淀粉酶的最适pH为7.2 ～8.2,其他器官的最适pH均为7.2.各部位在最适温度和pH下,脂肪酶酶活力顺序为肠＞胃＞肝＞幽门盲囊,淀粉酶酶活力顺序为肠＞幽门盲囊＞胃＞肝;蛋白酶在最适温度和pH下的酶活力顺序分别为肠＞胃＞肝＞幽门盲囊、肠＞胃＞幽门盲囊＞肝.
The effect of reaction temperature and pH on the activities of 3 main digestive enzymes （ prote- ase, lipase, amylase） of Cromileptes altivelis were studied using enzymology analysis method. The results show that： the activities of all digestive enzymes in Cromileptes altivelis increased at first and then decreased with temperature and pH increasing in the designed temperature and pH ranges. In stomach, the optimal temperature for protease was 40 - 45 ℃ , whereas all in intestine, pyloric caecum and liver were 40 ℃. The optimum temperature for lipase in stomach was 35 ℃, the optimum temperature for the pyloric caecum was 40 - 45 ℃, the others were all 40 ℃ respectively. The optimum temperature for amylase in stomach was 40 ℃, The others were all 35 ℃ respectively. The optimum pH for protease in stomach was 3.2, the optimum pH values in intestine, pyloric caecum and liver were all 8.2. The optimum pH values for lipase in stomach and liver were 7.2, 8.2, the others were all 6.2 respectively. The optimum temperature for amylase in stomach was pH 7.2 - 8.2 and others were all 7.2. Under optimum tempera-ture and pH, the activities of lipase in different organs was in the order of anterior intestine 〉 stomach 〉 liver 〉 pyloric caecum, and the activities of amylase were intestinal 〉 the pyloric caecum 〉 stomach 〉 liver. Under optimum temperature and pH , the order of protease was intestine 〉 stomach 〉 liver 〉 py- loric caecum and intestine 〉 stomach 〉 pyloric caecum 〉 liver.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第3期95-100,共6页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广东省海洋渔业科技推广专项资助项目(A201200E02
B201201B17)
关键词
驼背鲈
消化酶活力
温度
PH
Cromileptes altivelis
digestive enzymes activity
temperature
pH
