摘要
目的对河北地区烧伤患者创面分离的120株金黄色葡萄球菌进行mecA和SCCmec检测分析MRSA耐药机制,为临床合理用药提供依据。方法在2009~2013年收治的烧伤患者创面中分离的120株金黄色葡萄球菌,采用头孢西丁纸片法进行MRSA筛选,对mecA基因,SCCmec和spa基因进行PCR扩增以及分型。结果 120株金黄色葡萄球菌中有74株为MRSA,占61.7%。药敏试验显示,MRSA对16种临床常见抗生素耐药率,超过85%的有7种,依次为苯唑西林（98.6%）,青霉素（96.0%）,环丙沙星（94.6%）,阿莫西林和头孢唑林（89.2%）,亚胺培南（87.8%）,庆大霉素（85.1%）,另有1株对万古霉素耐药。结论本组金黄色葡萄球菌MRSA检出率较高,并表现出较高的耐药性。MRSA具有的多重耐药性mecA基因密切关系。
Objectives To detect the mecA gene and the staphylococcal cassette chromosome mec（SCCmec）in 120strains of Staphylococcus aureus isolated from patients with burns in the Hebei area and to ascertain the mechanism of drug resistance of methicillin-resistant S.aureus（MRSA）in order to provide a basis for rational drug use in clinical practice. Methods One hundred and twenty strains of S.aureus were isolated from patients with burns in the Hebei area from 2009-2013.These strains were screened for MRSA using cefoxitin disk diffusion.The mecA gene,SCCmec,and Spagene were amplified using PCR and genotyped. Results Of 120strains of S.aureus,74were MRSA,accounting for 61.7%of the isolates.Drug susceptibility testing indicated that MRSA strains had resistance of over 85%to 7of 16commonly used antibiotics.MRSA was resistant to oxacillin（98.6%）,penicillin（96%）,ciprofloxacin（94.6%）,amoxicillin and cefazolin（89.2%）,imipenem（87.8%）,and gentamicin（85.1%）.Only 1strain was resistant to vancomycin. Conclusion MRSA was prevalent in the samples analyzed and these strains were highly drug-resistant.The multi-drug resistance of MRSA strains is closely related to the mecA gene of MRSA.
出处
《中国病原生物学杂志》
CSCD
北大核心
2014年第4期376-380,F0003,共6页
Journal of Pathogen Biology
