摘要
苹果茎痘病毒(Apple stem pitting virus,ASPV)是一种严重危害果树生产的潜隐性病毒。基于前期研究所获得的6个ASPV中国分离物CP基因的核苷酸序列,结合GenBank中报道的序列,对ASPV种群遗传多样性特点进行分析。应用MEGA 4.0软件分析了不同地理来源、不同寄主来源组内和组间的各分离物CP基因的遗传多样性,表明ASPV种群遗传变异与寄主来源、地理分布不存在明显的相关性。CP编码区仍处于净化选择中,亚洲的分离物、梨分离物承受着较高的选择压力。DnaSP软件分析显示,ASPV经历了明显的种群扩张,并有继续扩展蔓延之势。因而有必要进一步加强病毒检测及种苗脱毒,严格控制带毒种苗传播。
Apple stem pitting virus（ASPV） is one of the most important latent viruses that infects fruit trees. Based on the nucleotide sequence of coat protein（CP） gene from 6 Chinese isolates and other isolates from GenBank, the characteristics of genetic polymorphism of ASPV population were analyzed. The within-population and between-population nucleotide diversities of CP gene among different geographical and hosts origins were analyzed with MEGA 4.0. The results indicated that there were not various relativities between the genetic polymorphism and the geographical and hosts origins. The variation of CP gene was located in purifying selection, and the Asian isolates and pear isolates of ASPV had higher pressure of selection. The analysis of DnaSP software showed that ASPV suffered various population dilatation and the dilatation would continue to develop. Therefore, it need further enhance the detection of virus and elimination of virus for seedlings and strictly control the spread of seedlings infected by ASPV.
出处
《广东农业科学》
CAS
CSCD
北大核心
2014年第6期93-95,共3页
Guangdong Agricultural Sciences
基金
中央高校基本科研业务费(DL13EA06-3)
关键词
苹果茎痘病毒
种群
核苷酸多样性
Apple stem pitting virus
population
nucleotide diversities
