摘要
目的：分析小儿肺炎支原体（MP）感染的血清检测方法。方法选取在黄冈市进行血液MP检测的呼吸道感染患儿400例，对其采取咽拭子肺炎支原体DNA聚合酶链反应（MP-DNA-PCR）和血清肺炎支原体-免疫球蛋白M抗体（抗MP-IgM），并根据不同季节、年龄段的检出率进行分析比较。结果MP-DNA-PCR法检测的阳性率明显高于抗MP-IgM法，差异有统计学意义（P＜0．05）；8～12岁儿童检测的阳性率比其他年龄段儿童更高，各个年龄段患儿MP-DNA-PCR法阳性率均高于抗MP-IgM法，差异有统计学意义（P＜0．05）；第一季度以及第四季度阳性率明显高于第二、三季度，各个季度MP-DNA-PCR法阳性率均高于抗MP-IgM法，差异有统计学意义（P＜0．05）。结论MP-DNA-PCR法优于血清抗MP-IgM法，可作为标准检测方法为临床诊断服务。
Objective To analyze the serum markers in children with infection of Mycoplasma pneumoniae （MP） .Methods Throat swabs and serum samples were collected from 400 children with infection of MP ,and detec-ted for MP DNA by polymerase chain reaction（PCR） and anti-MP-IgM antibody .All results were analyzed according to seasons and ages .Results The positive rate of MP-DNA-PCR detection was significantly higher than anti-MP-IgM detection（P〈0 .05） .Of the both two methods ,the positive rate in children at age of 8-12 year-old was higher than those in children at other ages ,and of children at different ages ,the positive rates of MP-DNA-PCR detection were significantly higher than anti-MP-IgM detection（P〈 0 .05） .The positive rates of the first and fourth quarter were significantly higher than those of the second and third quarter ,and of each quarter ,the positive rates of MP-DNA-PCR detection were significantly higher than anti-MP-IgM detection（P〈 0 .05） .Conclusion MP-DNA-PCR detection might be superior to serum anti-M P-IgM detection ,and the former could be used as a standard test for clini-cal diagnosis .
出处
《检验医学与临床》
CAS
2014年第9期1167-1168,共2页
Laboratory Medicine and Clinic
基金
湖北省黄冈市卫生局一般规划项目(HW-2009BNO.017)
关键词
小儿
肺炎
支原体感染
血清
children
pneumonia
mycoplasma infection
serum
