摘要
目的探讨IL-4对小鼠骨髓树突状细胞(DC细胞)表面分子CD11c、CD80、CD86表达的影响及其意义。方法对照组(n=5只,30孔)应用20 ng/ml GM-CSF,实验组(n=5只,30孔)应用20 ng/ml GM-CSF+20 ng/ml IL-4分别刺激小鼠骨髓细胞生长,隔日进行细胞换液,观察并对比细胞形态学变化,流式细胞仪测定DC细胞表面相关分子表达。结果实验组诱导小鼠骨髓细胞第7日观察可见DC细胞形态,对照组第7日未发现明显DC细胞形态,流式细胞仪测定实验组CD11c(0.546±0.289)、CD80(0.506±0.085)、CD86(0.562±0.260)表达分别较对照组CD11c(0.236±0.058)、CD80(0.279±0.096)、CD86(0.237±0.070)表达明显增高(P<0.05)。结论 IL-4可以促进小鼠骨髓DC细胞表面分子CD11c、CD80、CD86表达,促进DC细胞分化成熟。
Objective To explore the efficacy of IL-4 on the expression of phenotype of CD11c, CD80, CD86 and its underlying meaning in cultured dendritic cells （DC）. Methods On the base of the routine culture, both the control group （n=5, 30 holes） and the test group （n=5, 30 holes） of mouse bone marrow cells were added with 20 ng/ml GM-CSF. In addition the test group was further treated with 20 ng/ml of IL-4. After 7 days, both groups were observed under microscope and CD11c, CD80, CD86 were measured with flow cytometry. Results At the 7 days, the DC cells were found under microcopy in the test group but not in the control group. Flow cytometry demonstrated the cell phenotype of CD11c （0.546 ±0.289）, CD80（0.506±0.085） and CD86（0.562±0.260） expression in test group were higher than that of the control group, which were CD11c （0.236±0.058）, CD80 （0.279±0.096） and CD86 （0.237±0.070）, respectively （P〈0.05）. Conclusions IL-4 could effectively promote the phenotype molecule expression of CD11c,CD80,CD86, and thus stimulate the differentiation and maturation of bone marrow cells to DC cells.
出处
《中华临床医师杂志（电子版）》
CAS
2014年第5期74-76,共3页
Chinese Journal of Clinicians(Electronic Edition)
