摘要
目的 观察艾迪注射液在放射性肺损伤中的防治作用,并探讨其相关机制.方法 将SPF级C57BL/6小鼠120只随机分为4组：空白对照组,照射＋艾迪组,照射＋甲泼尼龙组和照射组,每组30只.给予小鼠单次12 Gy胸部照射以建立放射性肺损伤模型,照射前2 h及以后每日腹腔注射艾迪及甲泼尼龙.分别于照射后1,24,72 h及1,2,4,8,16和24周处死小鼠,取左肺固定做组织切片,行苏木精-伊红（HE）染色观察病理变化、Masson染色观察胶原形成和免疫组化检测转化生长因子β1（TGF-β1）蛋白含量,取右肺行Western-blot测定TGF-β1蛋白含量,Real-time 聚合酶链反应（PCR）测定TGF-β1mRNA含量.结果 小鼠在接受照射后于1～2周出现放射性肺炎表现,TGF-β1mRNA和蛋白含量升高,但在给予艾迪注射液和甲泼尼龙处理组中TGF-β1mRNA和蛋白相对含量较照射组少,差异有统计学意义（P＜0.05）,照射＋艾迪组和照射＋甲泼尼龙组差异无统计学意义.在照射后16及24周观察到纤维形成增加,但照射＋艾迪组和照射＋甲泼尼龙组较照射组少.结论 艾迪注射液和甲泼尼龙可以下调因照射引起的TGF-β1mRNA和蛋白含量上升,二者在此作用上未观察到差别,这可能是其减轻放射性肺损伤的关键.
Objective To study the effect of aidi injection on radiation-induced lung injury and the probable Mechanism. Methods One hundred and twenty C57BL/6 mice were exposed to either sham radiation or single fraction of12 Gy delivered to the thorax. Four groups were defined as follows：in normal control group,the mice received neither radiation nordrug;in aidi injection＋radiation group,the mice received radiation and aidi injection;in Solu Medrol＋radiation group,the micereceived Solu Medrol;in radiation group,the mice were exposed to radiation. Aidi or Solu Medrol were injected intraperitoneally 2hbefore the radiation and daily after the radiation. Mice were sacrificed 1,24,72 hours,1 ,2 ,4,8,16 and 24 weeks after radiation.The left lungs were fixed and sectioned. The sections were stained by haematoxylin and eosin, masson stain andimmunohistochemical stain for transforming growth factor β1（TGF-β1 ）. The right lungs were collected to detect TGF-β1 mRNAexpression by reverse transcipiase quantitative real-time polymerase chain reaction （RT-PCR） and TGF-β1 protein expression byWestern blotting. Results Pneumonitis developed 1 to 2 weeks after radiation. Their TGF-β1 mRNA and protein expressionlevels in radiation-treated mice were significantly higher than those in normal control group （both P〈0. 05）. But the effects werereversed by aidi and Solu Medrol treatment （P〈0. 05）. The TGF-β1 mRNA and protein expression levels were not significantlydifferent between aidi and Solu Medrol treatment. Conclusion Both aidi injection and Solu Medrol can significantly down-regulate TGF-β1 mRNA and protein expression levels in the lungs of mice with radiation-induced lung injury. There is nosignificant difference in the effect between aidi injection and Solu Medrol.
出处
《医药导报》
CAS
北大核心
2014年第2期184-188,共5页
Herald of Medicine
关键词
艾迪注射液
肺损伤
放射性
转化生长因子Β1
Aidi injection
Radiation-induced lung injury
Transforming growth factor-1
