摘要
目的 建立高效液相色谱法测定人血浆中去铁酮（DFP）含量.方法 Kromasil C18色谱柱（4.6 mm×200 mm,5 μm）,流动相：乙腈-50 mmol·L-1磷酸二氢钾（含5 mmol·L-1庚烷磺酸钠,1%三乙胺）＝11：89（用磷酸调至pH1.3）,检测波长：278 nm,柱温：25 ℃,进样量：20 μL,流速：1 mL·min-1.结果 DFP在0.5～80.0 mg·L-1浓度范围内线性良好（r＝0.996 5）,最低检出浓度为0.5 mg·L-1.高、中、低3种浓度样品绝对回收率＞85.6%,方法回收率＞89.9%,日内精密度RSD在1.61%～3.70%,日间精密度RSD在1.84%～4.81%.结论 该方法操作简便易行,快速准确,灵敏度高,专属性强,适用于DFP的人体血浆浓度测定.
Objective To establish a high performance liquid chromatography （HPLC） method for the determination ofdeferiprone in human plasma. Methods The analytical column was a Kromasil C18 column （4. 6 mm×200 mm, 5 μm）. Themobile phase was a mixture of acetonitrile and buffer of 50 mmol·L-1 potassium dihydrogen phosphate （containing 5 mmol·L-1sodium heptanesulfonate and 1% triethylamine） （1189, pH adjusted to 1. 3 with phosphoric acid）. Detection wave-length wasset at 278 nm. The column temperature was 25 ℃. The injected volume was 20 μ L. Flow rate was 1 mL·min-1 . Results Thecalibration curve of deferiprone were linear in the range of 0. 5-80. 0 mg·L-1（r =0. 996 5）, and the lower limit of detection was0. 5 mg·L-1 . The absolute recoveries of deferiprone at low, medium and high concentrations were more than 85. 6%, and themethod recoveries were more than 89. 9%. The intraday coefficients of variation were 1. 61% - 3. 70%, and the interdaycoefficients of variation were 1. 84% -4. 81%. Conclusion The method is simple, quick, accurate, sensitive, and specific,and can be used in detecting deferiprone concentration in human plasma.
出处
《医药导报》
CAS
北大核心
2014年第2期164-167,共4页
Herald of Medicine
基金
广西科技厅自然科学基金面上项目(2012GXNSFAA053145)
广西卫生厅自筹经费科研课题(Z2011347)
