摘要
试验在田间种植条件下研究转BADH基因耐盐碱玉米在不同生育期对盐碱土和普通耕土中三种土壤酶活性的影响。结果表明,在整个生育期内,两种类型土壤的土壤脲酶活性均呈现"升高-降低-升高"趋势,蔗糖酶均呈现"升高-降低"趋势,而脱氢酶在盐碱土壤中呈现"升高-降低"趋势,在普通耕土中始终是降低的;盐碱土壤的脲酶和脱氢酶高于普通耕土,蔗糖酶活性低于普通耕土;与非转基因受体对照相比,转基因玉米株系种植在盐碱土中促进土壤脲酶活性,抑制土壤蔗糖酶活性,拔节期时达到差异显著(P<0.05)和极显著水平(P<0.01),分别提高26.77%和降低53.73%,对土壤脱氢酶起促进作用;在普通耕土中,转基因玉米种植对土壤脲酶活性有促进作用,对土壤脱氢酶起抑制作用,分别在拔节期和吐丝期时达到差异显著(P<0.05),分别提高46.95%和降低28.79%,对土壤蔗糖酶起抑制作用。
The effects of genetically modified salt-tolerant maize with the gene BADH on soil urease, sucrase and dehydrogenase activity at different growth stages in saline-alkali soil and common soil under the filed condition. The results showed that during the whole growth period, urease activity increased at first and then decreased and increased finally in two types of soil.Sucrase activity increased at first and then decreased, dehydrogenase activity increased at first and then decreased in saline-alkali soil, and always decreased in common soil. Compared to common soil, the urease and dehydrogenase activity of saline-alkali soil were higher and sucrose activity was lower. The planting of transgenic maize in saline-alkali soil increased （26.77%） urease and dehydrogenase activity and decreased （53.73%） sucrose activity compared with non-transgenic maize, and the differences reached the significant level （P〈0.05） and the great significant level （P〈0.01） separately in jointing stage. In the common soil, it had an increased （46.95%） effect on urease activity and a decreased （28.79%） effect on dehydrogenase activity. The differences reached the significant level （P〈0.05） in jointing and silking stage,respectively. And the sucrose activity decreased in common soil.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2014年第4期25-29,共5页
Journal of Northeast Agricultural University
基金
国家转基因生物新品种培育重大专项(2014ZX08011003)
东北农业大学科学研究基金资助(2012RCB21)
