摘要
目的：建立高效液相色谱法同时测定人血浆中异烟肼、乙酰异烟肼和吡嗪酰胺的浓度。方法：色谱柱为Inertsil ODS-SP（4.6 mm×150 mm,5μm）,以甲醇∶0.05 mol·L-1磷酸二氢钾（三乙胺调pH 5.6）为流动相,柱温：40℃,流速：1.0 mL·min-1,进样体积：20μL,检测波长：268nm。结果：3组分在线性范围内与峰面积线性关系良好（r2〉0.9940）,日内、日间精密度RSD〈10%,平均回收率在80%~120%。结论：本方法专属性好,样品处理简单,符合生物样品分析要求。
Objective：To determine the concentrations of isoniazid,acetylisoniazid and pyrazinamide in human plasma by RP-HPLC.Methods：The HPLC method was carried out on an Inertsil ODS-SP（4.6× 150 mm,5 μm） column with 0.05 mol·L-1KH2PO4（adjusting PH to 6.0 with triethylamine）-methanol as mobile phase.The column temperature was 40 ℃,the flow rate was 1.0 mL·min-1and the detection wavelength was 268 nm.Results：All calibration curves had good linearity（r2＆gt;0.99） within their tested ranges.The intra-and inter-day precision was within 10%,the extraction recovery was between 80% and 120%.Conclusion：The method is sensitive,simple and rapid.It is suitable for analyzing the drugs in biological samples.
出处
《药学与临床研究》
2014年第2期112-114,共3页
Pharmaceutical and Clinical Research
基金
南京市科委药学基金(常州四药临床药学研究基金)
编号:2013YX015
南京市医学科技发展重点项目(编号:ZKX13041)
