摘要
在pH 3.6的BR缓冲溶液中,鸟苷与金纳米间相互作用,可降低金纳米粒子表面之间的静电斥力,从而改变溶液的吸光度。当鸟苷的浓度在1.75×10^-8-6.27×10^-6mol/L时,与体系ΔA630/A530形成良好的线性关系,其回归方程为：ΔA630/A530=1.33c×10^-6mol/L＋0.017 5,相关系数r=0.997。检出限为5.26×10^-9mol/L。相对标准偏差（RSD）为1.97%-3.26%,回收率为96.2%-98.9%。该法具有操作简单、快速、灵敏度高的优点。
In the Britton-Robinson buffer solution of pH 3. 6,guanosine combined with gold nanoparticles, resulting in the absorbance change of the solution. There is a good linear correlation existed between the absorbance change（ ΔA） and the concentration of 8-OHdG in the range of 1. 75 × 10^- 8- 6. 27 × 10^- 6mol / L. The linear regression equation is： ΔA630/ A530= 1. 33c × 10^- 6mol / L ＋ 0. 017 5 and the correlation coefficient is 0. 997. The detection limit is 5. 26 × 10^- 9mol / L,the relative standard deviations（ RSD） were 1. 97% -3. 26%. The proposed method has also been successfully applied for the determination of guanosine in real samples with the recoveries of 96. 2% - 98. 9%. The new method was simple, rapid and sensitive.
出处
《应用化工》
CAS
CSCD
2014年第4期743-745,共3页
Applied Chemical Industry
基金
湖南省科技厅科技计划资助项目(2010SK3039)
关键词
金纳米
鸟苷
分光光度法
gold nanoparticles
guanosine
spectrophotometry
