摘要
目的:探讨降脂颗粒是否对小鼠非酒精性脂肪性肝炎(NASH)有治疗作用及其机制。方法:利用蛋氨酸-胆碱缺乏(MCD)饲料饲养C57BL/6小鼠6周,建立NASH模型,药物组同时予以降脂颗粒干预。肝组织HE染色观察病理性变化,检测血清转氨酶以明确肝功能情况,并检测血清抵抗素水平及肝组织核因子-κB(NF-κB)表达水平。结果:MCD饮食模型小鼠出现肝脏严重脂肪变性和炎症坏死,血清转氨酶显著升高(P<0.01)。降脂颗粒组小鼠肝组织的脂质沉积明显减轻,炎性细胞浸润和坏死灶较少,血清转氨酶AST较模型组下调(P<0.05)。模型组小鼠血清中抵抗素含量较正常组小鼠明显升高(P<0.05),降脂颗粒组较模型组其含量显著下调(P<0.01)。NF-κB表达在模型组升高(P<0.05),在降脂颗粒组被改善(P<0.05)。结论:降脂颗粒可有效改善MCD饮食诱导的小鼠NASH病理损伤,对脂肪性细胞因子抵抗素及其相关分子的调节是其药效作用靶点之一。
Objective： To study the effect of Jiangzhi Granule on non-alcoholic steatohepatifis （NASH） mice model and explore the underlying mechanism. Methods： Methionine and choline deficient （MCD） diet was used to feed C57BL/6 for 6 weeks to establish NASH model. Meantime, Jiangzhi Granule was administrated intragastricly everyday for the intervention group. Liver histopathology was observed through HE stain and liver injury was determined by serum aminotransferase ALT and AST. Serum resistin concentration was measured by Bio-Plex 200 and the relative assay kit. And liver NF-κB protein expression was determined through Western Blot. Results： Compared with normal group, model group showed massive steatosis in liver lobules with inflammatory necrosis and hepatocyte ballooning appeared in central lobules, which were improved in the intervention group. Levels of serum ALT （P〈0.01）, AST activity （P〈0.01） and resistin content （P〈0.05） increased dramatically in model mice, while AST activity （P〈0.05） and resistin level were down-regulated in the intervention mice. NF-κB protein was expressed higher in model group than the control （P〈0.05）, which was reduced in Jiangzhi Granule treating mice obviously （P〈0.05）. Conclusion： Jiangzhi Granule could attenuate the pathogenesis of MCD induced NASH in mice. And the regulation of resistin level is one target of the underlying mechanism.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第5期1674-1677,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金(No.81202667
No.81273727)
上海市教育委员会科研创新项目(No.12ZZ119
No.ZZszy12062
No.E03008)
上海市浦东新区卫生局项目(No.PW2012A-40)~~
