摘要
植物miRNA能够参与盐胁迫下基因表达的调控。在盐胁迫条件下盐生植物花花柴miR398（KcmiR398）呈现显著性差异表达，为了探讨rniR398的靶基因及其对靶基因的调控方式，通过生物信息学分析（http：／／plant—grn．noble．Org／psRNATarget／），预测KcmiR398的靶基因分别为Cu／Zn超氧化物歧化酶（SOD）基因CSDl和CSD2。利用RACE技术从花花柴中克隆到CSDl和CSD2基因全长序列，分别命名为KcCSDl和KcCSD2。qRTPCR结果表明：（1）KcmiR398在盐胁迫的花花柴茎中上调表达，在新叶中下调表达；KcCSDl在盐胁迫的老叶中上调表达，而在老茎和根中下调表达；KcCSD2在盐胁迫的老茎和根中上调表达。（2）300mmol／I，NaCl胁迫24~48h，KcmiR398与对照无显著差异；KcCSDl的表达为对照的3倍以上，而KcCSD2的表达没有显著差异。研究表明，花花柴的KcmiR398和KcCSDl、KcCSD2有组织差异性表达，盐胁迫可以影响KcmiR398和KcCSDl、KcCSD2基因的表达以适应盐胁迫产生的氧化危害。
Some plant miRNAs are involved in gene expression regulation under salt stress. KcrniR398, a miRNA from halophytic Karelinia caspica, presented a significant differential expression under salt stress. In order to investigate the target genes of KcmiR398 and its regulatory effect to the target genes,we per- formed online bioinformatics analysis （http：//plantgrn. noble. Org/psRNATarget/）, and Cu/Zn superox- ide dismutase （SOD） genes CSD1 and CSD2 were predicted as the target genes o{ KcrniR398. Full length sequences of CSD1 and CSD2 （named KcCSD1 and KcCSD2 respectively） were cloned by using RACE technology, qRT-PCR results showed that： （1）The expression of KcmiR398 was up-regulated in the stems of the salt-stressed K. caspica,but down-regulated in the new leaves;The expression of KcCSD1 was up- regulated in the old leaves of the salt stressed plant, but clown-regulated in the old stems and roots;The ex- pression of KcCSD2 was up-regulated in both old stems and roots of the stressed plant. （2）When plant stressed with 300 mmol/L NaC1 for 24-48 h,the expression of KcmiR398 showed no significant difference compared to the control;the expression of KcCSD1 was more than 3-fold of that of control;whereas the ex- pression of KcCSD2 showed no significant difference with salt treatment. These results indicated that the expression of KcmiR398,KcCSD1 and KcCSD2 were tissue differential,and KcCSD1 and KcCSD2 was influenced tion caused by salt stress. by salt stress,which makes K. caspica the expression of KcmiR398, resist to the damage of oxida tion caused by salt stress.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第4期682-688,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30960035)
国家“973”计划前期研究专项资助(2012CB722204)
