摘要
目的持续静脉给予大鼠肾上腺素模拟应激导致的内耳缺血状态,观察给药前后大鼠听力及血清下丘脑-垂体-肾上腺轴(HPA轴)相关激素水平变化情况,探究两者之间的关系。方法 24只雄性Wistar大鼠按数字随机法分为3个组(对照组、8μg kg-1·min-1肾上腺素组、12μg kg-1·min-1肾上腺素组),每组8只。对照组给予生理盐水,后两组尾静脉持续给予肾上腺素3 h。各组分别于给药前、给药结束时进行听性脑干反应(Auditory Brainstem Response,ABR)检测听力。第2天复查听阈后处死,检测血清促肾上腺皮质激素释放激素(CRF)、促肾上腺皮质激素(ACTH)以及肾上腺皮质激素(GC)水平。结果与给药前比较,两肾上腺素组给药结束时ABR波Ⅰ、波Ⅲ听阈明显升高。两肾上腺素组给药结束时40 dB声强下波Ⅲ幅值较基础值明显下降。给药次日两肾上腺素组大鼠听阈以及幅值基本恢复,8μg kg-1·min-1组血清CRF、ACTH和GC水平较对照组均明显升高。结论静脉持续给予大鼠肾上腺素可以导致大鼠可逆性的听力损失,引起血清CRF、ACTH和GC含量升高。
Objective To study the changes of hearing and hormones of hypothalamic pituitary adrenal axis （ HPA axis ） in rats with internal ear ischemia induced by continuing intravenous administration of adrenalin. Methods 24 male Wistar rats were equally divided into 3 groups randomly, i.e. low dose group （ 8 ⅠLg · kg-1 ·min-1 ） , high dose group （ 12Ⅰg · kg-1 · min-1 ） and control group. Adrenalin was administrated systemically from the caudal vein for 3 hours. The control group was treated with equal volume of normal saline. Click evoked auditory brainstem response （ABR） was measured before administration, 3 hours after infusion and the next day. The concentration of cortieotropin releasing factor （ CRF ） , adrenocorticotropic hormone （ ACTH ） and glucocorticoid GC was measured by ELISA. Results After administration, the thresholds of waveⅠ , wave Ⅲ of the two adrenalin groups were significantly elevated, while the amplitudes of wave m at 40 dB of the adrenalin groups were significantly descended. The auditory thresholds and amplitudes recovered at the next day after administration. The serum concentration of CRF, ACTH, GC of the low dose group increased significantly over the control group. Conclusion Systemic continuing adrenalin administration can induce reversible hearing loss and increase in CRF, ACTH and GC in rats.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2014年第1期21-24,共4页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
北京市科委首都特色临床研究项目(2111107067311048)
