摘要
目的探讨丙泊酚对肺癌细胞增殖和凋亡的影响及其相关的分子机制。方法将HCC827肺癌细胞接种于培养板中培养,密度为1×106/mL,采用随机数字表法,将其分为5组:正常组(C组),脂肪乳组(10μg/mL,E组),低剂量丙泊酚组(1.5μL/mL,P1组),中剂量丙泊酚组(2.2μL/mL,P2组),高剂量丙泊酚组(3.2μL/mL,P3组)。丙泊酚处理后6、24、48h收集细胞,进行细胞增殖和凋亡检测;实验处理6h后,收集细胞,通过RT-PCR和Western blot进行Nrf2mRNA和蛋白的检测。结果与C组相比,E组细胞抑制率和细胞凋亡、Nrf2的mRNA和蛋白表达均无统计学差异(P>0.05);与C组和E组相比,P1、P2和P3组的细胞抑制率和细胞凋亡、Nrf2的mRNA和蛋白表达大量增加,差异具有统计学意义(P<0.05)。结论丙泊酚可以抑制肿瘤细胞的增殖,促进其凋亡,从而抑制肿瘤细胞的复发和转移。这一过程可能与激活细胞内的Nrf2表达密切相关。
Objective To investigate the effects of propofol on the proliferation and apoptosis of lung cancer cells as well as the related molecular mechanisms. Methods HCC827 cells were seeded in well plates with a density of 1 × 10^6 and then randomly divided into 5 groups., control group （group C）, intralipid group （group E）, low-dose propofol group （1. 5 μL/mL, group P1）, medium-dose propofol group （2.2 μL/mL, group P2）, and high-dose propofol group （3.2μL/mL, group P3）. At 6 h, 24 h and 48 h after propofol treatment, the cells were collected to detect their proliferation and apoptosis. At 6h after treatment, the cells were collected for the measurement of Nrf2 mRNA and protein by RT-PCR and Western blot. Results Cell inhibition rate （IR） and apoptosis as well as Nrf2 mRNA and protein expressions in group E did not differ significantly from those in group C （P〉0.05）. Compared with those in groups C and E, IR and apoptosis and Nrf2 mRNA and protein expressions were significantly increased in groups P1, P2 and P3 （P〈 0. 05）. Conclusion Propofol can inhibit the proliferation of cancer cells and promote cell apoptosis, thereby inhibiting the reoccurrence and metastasis of cancer cells probably via regulating the activation of Nrf2 expression.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2014年第3期361-363,384,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.81101409)~~
关键词
丙泊酚
肺癌
细胞增殖
细胞凋亡
propofol
lung cancer cell proliferation
cell apoptosis
