摘要
应用全细胞膜片钳技术在大鼠新鲜分离DRG神经元上观察多巴胺D1、D2受体选择性激动剂SKF38393和R(- ) NPA对GABAA 受体激活电流的影响。在 6 0个对GABA反应的细胞 ,预加SKF3839330s后观察到对GABA 激活电流幅值的影响 :多数为抑制 (5 3/ 6 0 ) ,少数为增强 (1/ 6 0 )和无明显作用 (6 / 6 0 )。另外对GABA敏感的 5 0个受检细胞 ,预加R(- ) NPA 30s后对GABA 激活电流幅值的影响也是多数为抑制 (38/ 5 0 ) ,少数为增强 (1/ 5 0 )和无明显作用 (11/ 5 0 )。SKF38393及R( ) NPA对GABA(10 -4 mol/L) 激活电流的抑制作用为浓度依赖性的。上述抑制可为胞内透析蛋白激酶抑制剂H7所翻转。
The experiment was performed on neurons freshly isolated from rat DRG by using whole cell patch clamp techniques When 60 cells responded to GABA were treated with SKF38393, a selective agonist of dopamine Dl receptor, it was found that except one cell which exhibited a slight potentiation( l0 0%,l/60), the inhibition by SKF38393 of GABA activated current was predominant (88 33%,53/60), while the remainders were of no effect( l 66%,6/60) In 50 neurons tested , when the neurons were treated with R(-) NPA prior to the application of GABA for 30sec, It was found that GABA activated membrane currents were inhibited (76%,38/50) , enhanced (2%,l/50) , no effected markedly (22%,11/50) The effect of SKF38393 and R(-) NPA was dependent on the concentration of SKF38393 and R(-) NPA By intracellular application of 10 -4 mol/L H7, a potent inhibitor of protein kinase , via micropipette the inhibition of SKF38393 and R(-) NPA was abolished apparently as compared with the control
出处
《基础医学与临床》
CSCD
北大核心
2001年第1期36-39,共4页
Basic and Clinical Medicine
基金
国家自然科学基金资助!(39370 2 39)
