摘要
目的:探讨淫羊藿总黄酮(Total epimedium flavonoids,TEF)对喹啉酸(Quinolinic acid,QA)诱导的人神经母细胞瘤细胞(SH-SY5Y)毒性的保护作用及其可能机制。方法:噻唑蓝(MTT)比色法测定QA的毒性作用;不同浓度淫羊藿总黄酮预处理SH-SY5Y细胞,再用QA共孵育,用MTT比色法测定细胞存活率;用Hochest33258染色观察细胞凋亡;用比色法测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。用流式细胞技术(Flow cytometry,FCM)检测细胞线粒体膜电位(Mitochondrial membrane potential,△Ψm)和细胞内Ca2+浓度([Ca2+]i)的变化。结果:淫羊藿总黄酮能提高细胞的存活率;使呈现细胞核聚集浓缩、致密浓染的凋亡细胞显著减少;提高SOD和GSH-Px活性,降低MDA含量;升高线粒体膜电位(△Ψm);降低细胞内钙离子浓度([Ca2+]i)。结论:淫羊藿总黄酮对QA诱导的SH-SY5Y细胞毒性有显著的保护作用。
Objective： To investigate the protective effects and its mechanisms of total epimedium flavonoids（ TEF） against Quindininc acid（ QA）-induced toxicity in SH-SY5Y cells. Methods： The MTT assay was used to detect the toxicity of QA and the cell viability of pretreatment of TEF and co-incubation with QA in SH-SY5Y cells; Cell apoptosis was observed by Hochest33258 staining; SOD and GSH-Px activities as well as MDA content were measured by colorimetric method; The mitochondrial membrane potential（ △Ψm） and intracellular free calcium concentration（ [Ca2 ＋]i） were monitored by FCM. Results： TEF increased the cell viability of SH-SY5Y cells,reduced apoptosis cells significantly,improved SOD and GSH-Px activities,decreased MDA content,increased the mitochondrial membrane potential and reduced intracellular free calcium concentration（ [Ca2 ＋]i）. Conclusion： TEF has significant protective effects against QA-induced toxicity in SH-SY5Y cells,the action mechanism may be associated with resisting oxidative stress,decreasing the [Ca2 ＋]iand up-regulating △Ψm.
出处
《中药材》
CAS
CSCD
北大核心
2013年第12期1978-1982,共5页
Journal of Chinese Medicinal Materials
