摘要
目的应用多重PCR方法扩增TCRα链全长序列,分析乳腺癌浸润淋巴结αT细胞受体谱型偏移。方法选取乳腺癌转移淋巴结,提取RNA后逆转录,多重PCR扩增TCRα链全长序列,构建重组质粒并测序,利用网上TCR资源分析序列。结果 2例患者各扩增出3个α链序列。乳腺癌TCRα限制性取用AV1、AV12亚家族。CDR3区序列分析增生T细胞克隆具有不同的氨基酸序列。结论乳腺癌浸润淋巴结扩增的TCR家族存在限制性取用,克隆性增生T细胞CDR3序列不同。
Objective Amplify the complete DNA sequence of TCR a - chain by Multi - PCR. To analyze the specific repertoire skewing in metastatic lymph node in patients with breast cancer. Methods The total RNA was extracted from lymph node metastasis and reverse transcriptase, Then to amplify the complete, DNA sequence of TCR α - chain by multi -PCR. The recombinant plasmids were sequenced and sequence analyze by using online TCR resources. Results There are 3 TCR - αchain CDS were obtained in two patients respectively. TCRα - chain presented specific repertoire skewing in metastatic lymph node. There were selected usage of AV1, AV12 of V alpha subfamily. The Clonal prolifera- tion T cells have different CDR3 amino acid sequences. Conclusion There were selected usage of alpha subfamily in metastatic lymph node in Patients with breast cancer. The sequences of CDR3 are different in different TCR clone prolif- eration.
出处
《医药论坛杂志》
2014年第4期9-11,共3页
Journal of Medical Forum
基金
河南省基础与前沿技术研究计划项目(102300410038)
关键词
乳腺肿瘤
T细胞受体
多重PCR
Breast neoplasm
T- cell antigen receptor（TCR）
Multi- PCR
