摘要
目的 揭示IPC后皮瓣中延迟相HSP70表达的动态变化 ,提出其规律及保护作用的最佳时间。方法 采用大鼠背部轴型皮管 ,制成活体原位IPC模型 ,检测皮管存活率 ,组织细胞超微结构 ,HSP70测定及统计学分析。结果 IPC组和IPC后延迟 48h断蒂组与对照组相比分别提高了皮瓣存活面积 1 2 6倍和 1 5 7倍 ;IPC后 48h组细胞合成功能旺盛 ,有大量的蛋白质合成并贮存 ;IPC后 48、72h组HSP70均较对照组升高 ,并且其表达以 48h较为明显。结论 IPC对鼠背轴型皮管具有保护作用 ,而对延迟断蒂组保护作用更佳 ,以 48h为最佳时间点。HSP70可能发挥着重要的作用。
Objective This study was to reveal the continuous changes of HSP70 and its regularity in the delayed protection of ischemic preconditioning axial flap in rats,then speculate the best time-piont of protection.Methods Animals were divided into three groups randomly(control、amputate after ischemia pretreatment and amputate delay after ischemia pretreatment).A left dorsal skin flap was crated in Wistar rat to ischemia precondition model to examine the survival rate of the skin flaps,electronic microscopic appearance,the contents of HSP70 at various times after they were given different treatment.Results The survival rate of amputate after ischamia pretreatment and amputate delay 48 h after ischemia pretreatment are 1.26 and 1.57 times as high as that of control. The celluar biosynthesis at 48 h after IPC is vigorous when there is a lot of proteins which were synthesized and stored. The contents of HSP70 at 48 h after IPC or at 72 h after IPC is much more than that of control,especially at the 48 h.Conclusions IPC can improve the survival rate of ischemia flap and amputate delay after IPC has more effect than IPC does.48 h may be the best time-point of IPC protection.It is suggest that the mechanism of IPC protection can be related to the function of HSP70.
出处
《中国急救医学》
CAS
CSCD
北大核心
2001年第3期139-141,共3页
Chinese Journal of Critical Care Medicine
