摘要
目的掌握生菜中肠杆菌污染分布情况,对生食生菜的风险分析提供科学依据。方法将50份生菜用自来水清洗后,用营养肉汤对其中肠杆菌进行增菌培养,平板分离后,再经生化鉴定,必要时进行血清试验,最终确定污染菌。重点关注风险隐患突出的致病性风险因子:沙门菌、致泻大肠埃希菌、志贺菌,同时关注罕见致病因子。结果此次实验中共分离得到6株肠杆菌科细菌,分别为大肠埃希菌、肺炎克雷伯菌肺炎亚种、产酸克雷伯菌、阴沟肠杆菌、粘质沙雷菌、摩氏摩根菌摩根亚种。结论此次调查未能从常规清洗后的生菜中发现肠道致病菌,但检测到多种条件致病菌,对人体健康存在一定的安全隐患,建议加强生菜从农田到餐桌的食源性致病菌监测,并对生食生菜提出安全指导。
Objective To master the Enterobacteriaceae contamination situation in fresh lettuce,in order to provide scientific basis for risk analysis of eating fresh lettuce. Methods Enterobacteriaceae in 50 washed lettuce samples were conducted enrichment culture in nutrient broth,then the pathogenic bacteria were determined through plate separation and biochemical identification. Serologic test was employed if necessary. Results Six Enterobacteriaceae strains named Escherichia coli,Klebsiella pneumoniae ssp pneumoniae,Klebsiella oxytoca,Enterobacter cloacae,Serratia marcescens and Morganella morganii ssp morganii were isolated from washed lettuce. Conclusion Pathogenic bacteria were not detected while multiple opportunistic pathogens were detected from the washed lettuce,suggesting foodborne pathogens monitoring on fresh lettuce should be strengthened from farm to dining table. In addition,safey instructions for eating fresh lettuce is necessary.
出处
《中国卫生检验杂志》
北大核心
2014年第7期1011-1013,共3页
Chinese Journal of Health Laboratory Technology
基金
四川省农业科学院青年基金项目(2013QNJJ-025)
关键词
生菜
肠杆菌
污染
监测
Lettuce
Enterobacteriaceae
Contamination
Monitor
