摘要
目的 探讨胃癌及癌前病变胃粘膜端粒酶 RNA与端粒酶活性检测及临床意义。方法 分别采用原位逆转录— PCR、端粒重复序列扩增法 (TRAP)检测 114例胃粘膜组织标本端粒酶 RNA与端粒酶活性 ,其中包括慢性浅表性胃炎 (CSG) 32例、不典型增生 (AH) 34例、胃癌 (GC) 4 8例。结果 原位逆转录— PCR检测胃粘膜活检标本的端粒酶RNA阳性率为 5 7.9% (6 6 / 114) ,显著高于 TRAP法检测端粒酶活性检出率 (4 4.7% ,5 1/ 114,P<0 .0 5 )。在不同胃粘膜病变中 ,AH及 GC组的端粒酶 RNA阳性率分别为 5 2 .9%、10 0 % ,而 CSG组胃粘膜中未检出端粒酶 RNA;AH及 GC组的胃粘膜端粒酶 RNA阳性率分别显著高于 CSG(P<0 .0 5 ) ,而 GC组端粒酶 RNA阳性率亦明显高于 AH组 (P<0 .0 5 )。端粒酶 RNA主要分布于胃粘膜癌细胞及癌前病变上皮细胞的胞核内。结论 端粒酶 RNA表达与胃癌的发生密切相关。原位逆转录— PCR检测胃粘膜端粒酶 RNA可能是较端粒酶活性更灵敏的生物学指标 。
Objective To study the expression of telomerase RNA in gastric cancer or its premalignant lesions and their clinical significance Methods Gastric biopsy specimens were taken from 114 patients who had chronic superficial gastritis (CSG. n=32), atypical hyperplasia(AH,n=34) and GC(n=48). Telomerase RNA was detected with in situ reverse transcription polymerase chain reaction (ISRT PCR) and telomerase activity with telomeric repeat amplification protocol(TRAP). Results The positive rate of telomerase RNA in gastric biopsy specimens was 57.9%(66/114), significantly higher as compared with that of telomerase activity(44.7%, P <0 05). Telomerase RNA expression in AH and GC was 52.9% and 100.0% respectively higher in GC than in AH ( P <0 05), and none in CSG(0.0%, P <0 05～0.01) Moreover, telomerase RNA was mainly located in the nuclei of gastric epithelial cells in patients with GC and premalignant lesions. Conclusion Telomerase RNA is closely related with GC. The detection of telomerase RNA may be helpful in predicting malignant change of gastric mucosa and in early diagnosis of gastric cancer. Furthermore, telomerase RNA might be a more sensitive biomarker as compared with telomerase activity.
出处
《肿瘤》
CAS
CSCD
北大核心
2001年第1期54-56,共3页
Tumor
